白细胞的分类计数虽然在临床上很重要。但是对白细胞严重减少症病人的外周血常不能得到满意的涂片,尤疑对这样很少的白细胞进行分类计数其结果是不准确的。白血病人常常需要治疗到呈现骨髓增生低下的程度,但即使白细胞明显减少,只要血涂片中仍见到幼稚细胞,可能是需要继续进行化疗的指征。此外,还有少数接受烃基药物或化疗结合放疗的病人发展为急性白血病,很多这样的病人在白血病转变时白细胞减少,因而在常规的外周血涂片中很难查到白血病细胞。本文介绍的这种简便的、由浓缩白细胞层(buffer coat layer)制备涂片的方法,不影响白 Although the white blood cell count is clinically important. However, patients with severe leukopenia often can not get satisfactory peripheral blood smear, especially for such a small number of white blood cells count the results of the inaccurate. People with leukemia often require treatment to the point where there is a low level of myeloproliferation, but even if leukocytes are significantly reduced, immature cells are still visible in the blood smear and may be an indication of the need for continued chemotherapy. In addition, there are a small number of patients receiving hydrocarbyl drugs or chemotherapy combined with radiotherapy to develop acute leukemia, many of these patients in the leukemia leukopenia, which is difficult in routine peripheral blood smear leukemia cells. This simple and easy method of preparing smears from the buffer coat layer does not affect the whiteness