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目的探讨聚腺苷二磷酸核糖聚合酶-1(poly ADP-ribose polymerase-1,PARP-1)mRNA在重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠肾脏中的表达及意义。方法48只Wistar大鼠按随机数字表法分为SAP组和假手术组(SO)组,分别于造模术后1、3、6及12h测定血清肌酐,观察胰腺和肾脏组织病理变化,并以RT-PCR法检测PARP-1mRNA在肾脏中的表达水平。结果SAP组大鼠术后血清肌酐逐渐升高,于3、6及12h明显高于SO组(P<0.05)。SAP组大鼠术后胰腺出现腺体破坏、腺泡坏死、出血、炎性细胞浸润等病理损害,且呈进行性加重;SO组各时相胰腺组织基本正常。SAP组大鼠术后出现肾小管上皮细胞变性、坏死、肾小球瘀血、缺血等改变,并随时间延长逐渐加重,其损伤程度在3、6及12h明显较SO组严重(P<0.05)。SO组大鼠肾脏组织仅表达少量PARP-1mRNA,而SAP组大鼠随病程延长肾脏组织中PARP-1mRNA表达逐渐增加,自3h时起明显高于SO组(P<0.01)。结论在SAP发病过程中,PARP-1mRNA的表达在肾脏组织中逐渐增加,PARP-1可能参与了SAP相关肾损伤过程。
Objective To investigate the expression of poly ADP-ribose polymerase-1 (PARP-1) mRNA in the kidney of rats with severe acute pancreatitis (SAP) and its significance. Methods 48 Wistar rats were randomly divided into SAP group and sham operation group (SO) according to random number table. Serum creatinine was measured at 1, 3, 6 and 12 h after operation. Pathological changes of pancreas and kidney were observed. The expression of PARP-1 mRNA in kidney was detected by RT-PCR. Results The postoperative serum creatinine in SAP group was significantly higher than that in SO group at 3, 6 and 12 hours (P <0.05). The pancreas of the SAP group showed glandular destruction, acinar necrosis, hemorrhage, infiltration of inflammatory cells and other pathological changes, and the progression was aggravated. The pancreas tissues in the SO group were basically normal. The changes of renal tubular epithelial cell degeneration, necrosis, glomerular blood stasis, ischemia and so on in SAP group were observed after operation, and gradually increased with the prolongation of time. The degree of injury in SAP group was significantly higher than that in SO group at 3, 6 and 12 hours (P < 0.05). In SO group, only a small amount of PARP-1 mRNA was expressed in the kidney of rats, whereas the expression of PARP-1 mRNA in the SAP group increased gradually with the prolongation of the course, and was significantly higher than that in the SO group at 3h (P <0.01). Conclusions The expression of PARP-1 mRNA gradually increases in the kidney tissue during the pathogenesis of SAP. PARP-1 may be involved in the process of SAP-related renal injury.