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采用间接免疫荧光法,检查5株单克隆抗体(下称单抗)与鼻咽癌组织和其他肿瘤组织的反应,并了解这些单抗所对应的抗原在肿瘤组织的定位及分布。结果表明,5株单抗均与鼻咽癌组织起反应,阳性率分别为H_3:78.38％,H_4:84.21％,H_5:91.43％,H_(12):67.57％,H_(13):70.27％,显示为膜荧光染色,在荧光表现形式上,鳞癌与腺癌不同。单抗与其他癌组织具有不同程度的交叉反应,而与肉瘤组织无反应。 另外,检查3株单抗(H_3,H_4、H_(12))与鼻咽癌病人及鼻咽部正常人的脱落细胞的免疫组化反应,发现均能与鼻咽癌病人的脱落癌细胞发生反应而不与正常的鼻咽部纤毛柱状上皮细胞和红细胞发生反应。
The indirect immunofluorescence method was used to examine the responses of 5 monoclonal antibodies (hereinafter referred to as monoclonal antibodies) to nasopharyngeal carcinoma tissues and other tumor tissues, and to understand the localization and distribution of the antigens corresponding to these monoclonal antibodies in tumor tissues. The results showed that 5 monoclonal antibodies reacted with nasopharyngeal carcinoma tissues. The positive rates were H_3: 78.38%, H_4: 84.21%, H_5: 91.43%, H_(12): 67.57%, H_(13): 70.27% respectively. Fluorescence staining of the membrane is shown. In the fluorescence manifestation, squamous cell carcinoma is different from adenocarcinoma. Monoclonal antibodies have different degrees of cross-reactivity with other cancerous tissues, but have no reaction with sarcoma tissues. In addition, the immunohistochemical reaction of 3 monoclonal antibodies (H3, H4, H12) with nasopharyngeal carcinoma and normal nasopharyngeal exfoliative cells was examined and found to be associated with exfoliative cancer cells in NPC patients. The reaction did not react with normal nasopharyngeal ciliated epithelial cells and red blood cells.