博尔纳病病毒(Borna disease virus,BDV)是一种非细胞溶解的单股负链嗜神经RNA病毒,可引起人畜共患病博尔纳病。目前研究表明,BDV几乎可感染所有的温血动物[1],但其流行现况尚不完全清楚。本研究采用改进的巢式逆转录酶聚合酶链反应(nRT-PCR)检测重庆三峡库区部分地域健康猪外周血 Borna disease virus (BDV) is a kind of non-cell lysis single-stranded negative neurotropic RNA virus that can cause zoonosis borna disease. The current research shows that BDV can infect almost all warm-blooded animals [1], but its prevalence is not fully understood. In this study, improved nested reverse transcriptase polymerase chain reaction (nRT-PCR) was used to detect peripheral blood of healthy pigs in some areas of the Three Gorges Reservoir in Chongqing