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人白细胞抗原G (humanleukocyteantigen G ,HLA G)属非经典的主要组织相容性复合体 (majorhistocompatibilitycomplex ,MHC)Ⅰ类分子 ,为自然杀伤细胞 (naturalkiller,NK)抑制性受体的识别配体 ,可以向NK细胞传递抑制性信号 ,从而抑制NK细胞的细胞毒作用 .为了研究HLA G对NK与靶细胞识别作用的影响机制 ,采用激光扫描共聚焦显微镜和流式细胞术对全长HLA G1的表达和功能进行了探讨 ,并对效靶识别过程中靶细胞 [Ca2 + ]i (胞内自由钙离子浓度 )进行了实时检测 .结果发现 ,全长的HLA G1表达于K5 6 2、JAR和CHO细胞的胞浆和胞膜上 ,它能够部分地抑制NK92的细胞毒作用 .NK92与CHO和GFP CHO细胞作用后 ,靶细胞 [Ca2 + ]i 出现了明显的升高 ,HLA G1的表达则抑制了靶细胞 [Ca2 + ]i的升高 .结果提示 :靶细胞[Ca2 + ]i 的升高是有效的细胞杀伤作用的必要条件 ,HLA G的免疫抑制功能可能与靶细胞胞内钙离子浓度升高的抑制作用有密切关系
Human leukocyte antigen G (HLA) is a non-canonical major histocompatibility complex (MHC) class I molecule, which is a recognition ligand for the natural killer (NK) inhibitory receptor. To inhibit the NK cell signal transduction, thereby inhibiting the cytotoxicity of NK cells.In order to study the mechanism of HLA G on NK and target cell recognition, using laser scanning confocal microscopy and flow cytometry expression of full-length HLA G1 (Ca2 +) i (intracellular free Ca2 + concentration) in target recognition.Results showed that full-length HLA G1 was expressed in K562, JAR and CHO It can partially inhibit the cytotoxicity of NK92.NK92 and CHO and GFP CHO cells, the target cells [Ca2] i showed a significant increase, while the expression of HLA G1 was inhibited The increase of [Ca2 +] i in target cells suggests that the increase of [Ca2 +] i in target cells is a necessary condition for effective cell killing. The immunosuppressive function of HLA G may be related to the intracellular calcium concentration Rise The inhibition is closely related to