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目的 :研究血管钠肽 (VNP)对人桡动脉 (humanradialartery,HRA)的舒张作用及其机制。方法 :采用离体血管灌流的方法 ,观察VNP对内皮完整和去内皮HRA的舒张作用 ,以及 8 Br cGMP、NPR A、B选择性抑制剂HS 14 2 1、鸟苷酸环化酶选择性抑制剂美蓝 (methyleneblue ,MB)和Ca2 + 激活K+ 通道 (Ca2 + activatedK+ channel,KCa)的选择性抑制剂TEA对这一过程的影响。结果 :VNP(10 10 ～ 10 -6mol/L)可剂量依赖性地舒张HRA ,该作用无内皮依赖性。 8 Br cGMP(10 -7～ 10 -3 mol/L)可模拟VNP的血管舒张效应。HS 14 2 1(2× 10 -5mol/L)或MB(10 -5mol/L)完全阻断VNP舒张作用。TEA(10 -3 mol/L)减弱VNP的舒血管作用。结论 :VNP对HRA具有不依赖内皮的舒张作用 ,它主要是通过作用于NPR A、B ,即钠尿肽的鸟苷酸环化酶耦联受体 ,升高细胞内的cGMP水平。KCa可能是其舒张作用的重要效应分子。为应用VNP防治血管移植术后的痉挛提供了重要的理论依据。目的 :研究血管钠肽 (VNP)对人桡动脉 (humanradialartery,HRA)的舒张作用及其机制。方法 :采用离体血管灌流的方法 ,观察VNP对内皮完整和去内皮HRA的舒张作用 ,以及 8 Br cGMP、NPR A、B选择性抑制剂HS 14 2 1、鸟苷酸环化酶选择性抑制剂美蓝 (methyleneblue ,MB)和Ca2 +
Objective: To investigate the vasodilation effect of vascular sodium peptide (VNP) on human radial artery (HRA) and its mechanism. Methods: The vasodilatory effects of VNP on endothelial integrity and endothelium-derived HRA were observed by means of ex vivo vascular perfusion, as well as selective inhibition of 8 Br cGMP, NPR A, B selective inhibitor HS 14 2 1 and guanylate cyclase The effect of TEA, a selective inhibitor of methylene blue (MB) and Ca2 + activated K + channel (KCa), on this process. Results: VNP (10 10 ~ 10 -6 mol / L) could relax HRA in a dose-dependent manner. This effect was not endothelium-dependent. 8 Br cGMP (10 -7 ~ 10 -3 mol / L) can simulate the vasodilatation effect of VNP. HS 14 2 1 (2 × 10 -5 mol / L) or MB (10 -5 mol / L) completely blocked the relaxation of VNP. TEA (10 -3 mol / L) attenuated the vasorelaxing effect of VNP. CONCLUSION: VNP has an endothelium-independent relaxation effect on HRA. It increases cGMP levels in cells primarily through guanylate cyclase-coupled receptors acting on NPR A, B, the natriuretic peptide. KCa may be an important effector of its relaxation effect. It provides an important theoretical basis for the application of VNP in the prevention and treatment of spasticity after vascular grafting. Objective: To investigate the vasodilation effect of vascular sodium peptide (VNP) on human radial artery (HRA) and its mechanism. Methods: The vasodilatory effects of VNP on endothelial integrity and endothelium-derived HRA were observed by means of ex vivo vascular perfusion, as well as selective inhibition of 8 Br cGMP, NPR A, B selective inhibitor HS 14 2 1 and guanylate cyclase Methylene blue (MB) and Ca2 +