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应用等离子体浸没离子注入装置对纯Ti表面进行氟离子注入,通过SEM和XPS分析研究材料的表面形貌和化学组成.将MG-63成骨细胞接种于氟离子注入前、后的Ti样品表面,采用激光共聚焦显微镜观察材料表面对成骨样细胞MG-63Ⅰ型胶原形成的影响;逆转录聚合酶链反应检测Ⅰ型胶原mRNA的表达;蛋白印迹法检测Ⅰ型胶原蛋白的表达.结果表明,氟离子注入后Ti样品表面新出现TiF_3;且MG-63细胞在其表面分别培养6,24和48 h后,Ⅰ型胶原的形成和表达均明显高于纯Ti表面.含氟表面改性层可提高Ti材料的生物相容性.
Fluorine ion implantation was performed on the pure Ti surface with a plasma immersion ion implantation apparatus, and the surface morphology and chemical composition of the material were investigated by SEM and XPS analysis.The MG-63 osteoblasts were seeded on the surface of the Ti sample before and after fluoride ion implantation , The effect of the material surface on the formation of osteogenic MG-63 type I collagen was observed by laser confocal microscopy, the expression of type I collagen mRNA was detected by reverse transcription polymerase chain reaction, and the expression of type I collagen was detected by Western blotting. , TiF_3 appeared on the surface of Ti sample after fluorine ion implantation, and the formation and expression of type Ⅰ collagen in MG-63 cells cultured on its surface for 6, 24 and 48 h respectively were significantly higher than that of pure Ti surface. The layer can improve the biocompatibility of the Ti material.