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目的以体外培养的大鼠肠黏膜微血管内皮细胞(RIMMVECs)为研究对象,探讨干扰素-γ(IFN-γ)对于体外培养的RIMMVECs表达细胞黏附因子-1(ICAM-1)和IFN-γ受体α(IFN-γRα)的影响。方法体外分离培养RIMMVECs,用不同浓度的IFN-γ对所培养的RIMMVECs进行诱导,通过荧光定量RT-PCR法和流式细胞术,从mRNA和蛋白水平检测活化后的RIMMVECs表达ICAM-1和IFN-γRα的情况。结果浓度为20μg/L和40μg/L的IFN-γ可以激活RIMMVECs,在mRNA和蛋白水平均能提高ICAM-1的表达,并且6 h时达到高峰;20μg/L的IFN-γ刺激RIMMVECs后,可使其上调表达IFN-γRαmRNA,但是蛋白表达量却随着刺激时间逐渐降低;而40μg/L的IFN-γ刺激RIMMVECs 2 h后,IFN-γRαmRNA达到峰值,6 h之后,其表达量呈下降趋势,IFN-γRα蛋白浓度也随之逐渐降低;10μg/L的IFN-γ对于ICAM-1和IFN-γRα的表达无影响。结论 IFN-γ可能是通过影响微血管内皮细胞(MVECs)表面IFN-γRα分子和ICAM-1的表达,从而调控和参与细胞免疫反应和炎症反应。
OBJECTIVE: To investigate the effect of interferon-γ (IFN-γ) on the expression of ICAM-1 and IFN-γ in RIMMVECs induced by in vitro culture of rat intestinal microvascular endothelial cells (RIMMVECs) Effect of body alpha (IFN-γRα). Methods RIMMVECs were isolated and cultured in vitro. The cultured RIMMVECs were induced by different concentrations of IFN-γ. The mRNA and protein levels of RIMMVECs were detected by RT-PCR and flow cytometry. The expression of ICAM-1 and IFN -γRα. Results IFN-γ at 20μg / L and 40μg / L could activate RIMMVECs and increase the expression of ICAM-1 at mRNA and protein levels, reaching the peak at 6 hours. After stimulating RIMMVECs with 20μg / L IFN-γ, IFN-γRαmRNA expression up-regulated, but the expression of IFN-γRαmRNA decreased gradually with the stimulation time; however, the expression of IFN-γRαprotein peaked at 2 h after stimulating RIMMVECs with 40μg / L IFN-γ, Trend, IFN-γRα protein concentration also will gradually decrease; 10μg / L of IFN-γ for ICAM-1 and IFN-γRα expression had no effect. Conclusion IFN-γ may regulate and participate in the cellular immune response and inflammatory response by affecting the expression of IFN-γRα molecule and ICAM-1 on the surface of microvascular endothelial cells (MVECs).