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目的:通过分析CD3高表达(CD3bright)T淋巴细胞(T细胞)群的表型特征,研究其在胃癌患者外周血中的数量变化,并探讨其在胃癌中的临床意义。方法:应用多参数流式细胞仪检测30例胃癌患者与35名健康对照者(健康对照组)外周血CD3、CD4、CD8、T淋巴细胞抗原受体αβ(TCRαβ)、T淋巴细胞抗原受体γδ(TCRγδ)的表达特征,检测CD3brightT细胞占T细胞的百分率,分析其与TCRγδ+T淋巴细胞间的关系。结果:临床检验中发现一群CD3brightT细胞,将其定义为CD3bright T细胞。该群细胞主要表达TCRγδ,且表型以CD4-CD8-为主,少部分表型为CD4-CD8+;CD3bright T细胞数与TCRγδ+T细胞数间显著相关(r=0.912,P<0.05)。胃癌患者的CD3bright T细胞占T细胞百分率[(3.46±1.65)%]低于健康对照组[(6.01±3.70)%],差异有统计学意义(P<0.05)。胃癌晚期(TNMⅣ期)患者外周血中CD3brightT细胞占T细胞百分率为(3.23±1.13)%(n=12),有低于TNMⅠ~Ⅲ期胃癌患者[(3.98±1.74)%(n=18)]的趋势,但差异尚无统计学意义(P>0.05)。结论:CD3brightT细胞主要为TCRγδ+T细胞,且其在胃癌患者外周血中占T细胞百分率降低,可能参与了胃癌的发生、发展。
OBJECTIVE: To investigate the phenotypic characteristics of CD3bright T lymphocyte (T) cell population and to study its clinical significance in gastric cancer. Methods: Multi-parameter flow cytometry was used to detect the expressions of CD3, CD4, CD8, TCRαβ, T lymphocyte antigen receptor in peripheral blood of 30 gastric cancer patients and 35 healthy controls (healthy control group) γδ (TCRγδ) expression characteristics of CD3brightT cells to detect the percentage of T cells, TCRγδ T lymphocytes and its relationship. Results: A panel of CD3bright T cells was identified in the clinical trial, which is defined as CD3bright T cells. The population of TCRγδ mainly expressed CD4-CD8- and some of the phenotypes were CD4-CD8 +. The number of CD3bright T cells was significantly correlated with the number of TCRγδ + T cells (r = 0.912, P <0.05). The percentage of T cells in CD3bright T cells in gastric cancer patients was significantly lower than that in healthy controls [(3.46 ± 1.65)% vs (6.01 ± 3.70)%, P <0.05). The percentage of CD3brightT cells in peripheral blood of patients with advanced gastric cancer (TNMⅣ) was (3.23 ± 1.13)% (n = 12), lower than that of patients with TNMⅠ ~ Ⅲ [3.98 ± 1.74% (n = 18) ] Trend, but the difference was not statistically significant (P> 0.05). Conclusion: CD3brightT cells are mainly TCRγδ + T cells, and their percentage of T cells in peripheral blood of patients with gastric cancer is decreased, which may be involved in the occurrence and development of gastric cancer.