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目的 探讨通心络对兔血管球囊损伤后血管平滑肌细胞 (VSMC)增殖和迁移的影响及其可能机制。方法 新西兰大白兔 6 4只 ,普食饲养 ,随机分为假手术组、对照组和通心络组 ,并分别于术前以及术后即刻及第 1、2、4周留取各组受损动脉标本和手术前后血液标本 ,分别行病理、电镜、RT PCR检验及血清NO浓度测定。结果 ( 1)术后对照组新生内膜明显增厚 ,内含大量增殖、迁移的VSMC ,VSMC呈典型的“合成型”表现 ,血管腔狭窄 ;通心络组以上改变较同期对照组显著减轻。 ( 2 )对照组新生内膜面积、内膜增生指数和增殖细胞核抗原 (PCNA)增殖指数均显著高于通心络组 (P <0 .0 1) ,但管腔面积和管腔狭窄指数均显著小于通心络组 ( P <0 .0 1)。 ( 3)对照组PCNA、基质金属蛋白酶 2 (MMP 2 )、MMP 3和血小板源性生长因子 BB(PDGF BB)的免疫组化及RT PCR表达均显著高于通心络组 ,而组织抑制因子 1(TIMP 1)的表达则相对低于通心络组。 ( 4 )术后对照组血清NO浓度显著低于通心络组 ( P<0 .0 5 )。结论 通心络能明显抑制球囊损伤后VSMC增殖和迁移 ,阻止内膜增生 ,防止血管狭窄等作用
Objective To investigate the effect of Tongxinluo on the proliferation and migration of vascular smooth muscle cells (VSMCs) after vascular balloon injury in rabbits and its possible mechanism. Methods Sixty-four New Zealand white rabbits were fed with normal diet and were randomly divided into sham-operated group, control group and Tongxinluo group. Rats in each group were sacrificed immediately before operation and immediately after operation Arterial specimens and blood samples before and after surgery were pathological, electron microscopy, RT PCR test and serum NO concentration determination. Results (1) The neointima of the control group was significantly thicker and contained a large number of proliferating and migrating VSMCs. The VSMC showed a typical “synthetic” appearance with narrowing of the vascular lumen. The changes in the Tongxinluo group were significantly lower than those in the control group . (2) The neointimal area, intimal hyperplasia index and proliferating cell nuclear antigen (PCNA) proliferation index in the control group were significantly higher than that in the Tongxinluo group (P <0.01), but both the lumen area and the stenosis index Significantly less than Tongxinluo group (P <0.01). (3) Immunohistochemistry and RT-PCR of PCNA, MMP 2, MMP 3 and PDGF BB in the control group were significantly higher than those in Tongxinluo group, while tissue inhibitor of metalloproteinase 1 (TIMP 1) expression was lower than Tongxinluo group. (4) The postoperative serum NO concentration in the control group was significantly lower than that in Tongxinluo (P <0.05). Conclusion Tongxinluo can significantly inhibit the proliferation and migration of VSMC after balloon injury, prevent intimal hyperplasia and prevent vascular stenosis