论文部分内容阅读
目的对比研究不同时期亚低温（ＭＨＴ３２℃±０．２℃）对局灶脑缺血再灌注损伤的作用。方法６４只雄性ＳＤ鼠被随机分成常温（ＮＴ）、缺血期亚低温（ＭＨＴｉ）、再灌注期亚低温（ＭＨＴｒ）、缺血期加再灌注期亚低温（ＭＨＴｉ＋ｒ）四组，并用改良Ｋｏｉｚｕｍｉ＇ｓ局灶脑缺血模型，分别观察了动物缺血３小时再灌注３小时过程中，缺血周边和核心区局部脑血流（ｒＣＢＦ）改变，再灌注３小时后血脑屏障（ＢＢＢ）破坏及再灌注７２小时后缺血梗塞灶体积。结果ＭＨＴｉ＋ｒ及ＭＨＴｉ均有改善缺血周边区再灌注后急性高灌注和继发低灌流及核心区持续低灌流、减轻血脑屏障破坏、减少缺血梗塞灶体积的作用。该作用尤以ＭＨＴｉ＋ｒ为明显。ＭＨＴｒ作用有限。结论ＭＨＴｉ＋ｒ对皮层的保护作用较ＭＨＴｉ好。由此可推知，局灶脑缺血再灌注损伤是个持续的过程，亚低温治疗不但要考虑开始时间，其持续时间对疗效具有同样重要价值
Objective To compare the effects of mild hypothermia (MHT32 ℃ ± 0.2 ℃) at different periods on focal cerebral ischemia-reperfusion injury. Methods Sixty-four male SD rats were randomly divided into four groups: normal temperature (NT), mild hypothermia (MHTi), reperfusion MHTr and ischemic reperfusion hypothermia (MHTi + r) ’S focal cerebral ischemia model, we observed the changes of regional cerebral blood flow (rCBF) in the peri-ischemic and core regions, and the blood-brain barrier (BBB) in the 3 hours after reperfusion. Destruction and infarct volume after 72 hours of reperfusion. Results Both MHTi + r and MHTi could ameliorate acute hyperperfusion, secondary hypoperfusion and continuous low perfusion in the core area after reperfusion in ischemic peripheral area, reduce the damage of blood-brain barrier and decrease the volume of ischemic infarction. This effect is especially evident for MHTi + r. MHTr limited role. Conclusion The protective effect of MHTi + r on cortex is better than that of MHTi. It can be inferred that focal cerebral ischemia-reperfusion injury is a continuous process, not only to consider the onset of mild hypothermia, its duration has the same value as the efficacy