具有抗炎生理活性的甾体化合物的合成,往往要引入C_(1-2)烯键(Δ~(1,2))。用生物化学方法引入Δ~(1,2)过程,需要一种快速、准确和灵敏的测定方法.Mader和Buck用比色法测定Δ~(1,4)酮醇甾体化合物含量,Ivashki将样品制成衍生物,然后用紫外吸收比方法测定Δ~(1,4)3-酮甾体化合物含量。然而,在甾体脱氢酶的研究中,至今仍常采用四氮唑盐作为电子受体的方法测定酶活力。 The synthesis of steroidal compounds with anti-inflammatory and physiological activity often introduces C 1-2 (a-1) (Δ ~ (1,2)). The biochemical method to introduce Δ ~ (1, 2) process requires a rapid, accurate and sensitive assay.Mader and Buck colorimetric determination of Δ ~ (1,4) ketol alcohol steroid compounds, Ivashki will The samples were made into derivatives, and then the content of Δ ~ (1,4) 3-ketosteroids was determined by UV absorption method. However, in the study of steroid dehydrogenase, the activity of tetrazolium salt as an electron acceptor has until now been measured.