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目的:研究生姜活性成分姜酚及其衍生物姜酚肟对慢性粒细胞系K562细胞的增殖活性、细胞周期及凋亡的影响。方法:采用CCK-8法检测姜酚和姜酚肟对K562细胞增殖活性的影响,PI单染检测其对K562细胞周期的影响,Annexin V-PI双染检测早期细胞凋亡,Hochest 33258染色观察细胞形态学的变化。结果:(1)姜酚作用于K562细胞48、72 h后,显著抑制K562细胞增殖,48 h的IC50值为15.75μg/mL,且具有时间及剂量依赖性。姜酚肟的抑制作用亦有时间及剂量依赖性,但较姜酚抑制作用弱。(2)姜酚15μg/mL组细胞周期阻滞于S期,姜酚肟作用组细胞周期与对照组比较无明显差异(P>0.05)。(3)姜酚和姜酚肟作用48 h后出现早期凋亡群,姜酚各浓度组间的早期凋亡率差异显著(P>0.05),随着药物浓度的增加凋亡率升高。(4)荧光显微间观察出现少量的细胞胞核固缩,核边集,出现凋亡小体。结论:姜酚和姜酚肟对K562细胞有显著的抑制作用,姜酚较姜酚肟抑制作用强,姜酚的抑制机制可能与影响细胞周期及诱导细胞凋亡有关,姜酚肟的抑制机制可能与诱导细胞凋亡有关。
OBJECTIVE: To investigate the effect of gingerol, an active constituent of graduate students, and its derivative gingivalis, on the proliferation, cell cycle and apoptosis of chronic myelogenous K562 cells. METHODS: CCK-8 was used to detect the effect of gingerol and ginger phenolphthalein on the proliferation of K562 cells. PI staining was used to detect the effect on K562 cell cycle. Annexin V-PI double staining was used to detect early apoptosis. Hochest 33258 staining Changes in cell morphology. Results: (1) Gingerol significantly inhibited the proliferation of K562 cells 48 hours and 72 hours after K562 cells treatment. The IC50 value at 48 h was 15.75 μg/mL, and it was time-and dose-dependent. The inhibitory effect of gingerol oxime was also time- and dose-dependent, but it was weaker than that of gingerol. (2) Gingerol 15μg/mL group cell cycle arrested in S phase, gingeroside action group cell cycle compared with the control group no significant difference (P> 0.05). (3) Early apoptotic populations appeared after 48 hours of gingerol and gingerol oxime, and the early apoptotic rates were significantly different among the groups (P>0.05). With the increase of drug concentration, the apoptotic rate increased. (4) A small amount of cellular nucleus pyknosis was observed between fluorescence microscopy, nuclear edge set, and apoptotic bodies appeared. Conclusion: Gingerol and Gingerol have a significant inhibitory effect on K562 cells. Gingerol has a stronger inhibitory effect than Gingerol. The inhibition mechanism of gingerol may be related to the influence of cell cycle and induction of cell apoptosis. May be related to induction of apoptosis.