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选择枯草芽孢杆菌、黑曲霉孢子和黑曲霉菌丝体等3种典型微生物制备生物样品,采用显微红外光谱仪对3种微生物样品在2.5—15μm波段的红外反射光谱进行测定,基于Kramers-Kronig(K-K)关系计算得到了微生物样品在6—14μm波段的复折射率.以水、蛋白质和核酸的红外吸收特性为基础,根据微生物样品的红外吸收谱线计算了微生物样品含水量.在3种微生物远红外波段复折射率和含水量的实测数据基础上,构建了生物颗粒在6—14μm远红外波段的复折射率模型,分析了模型的可靠性.模型的构建对发展生物样品的快速分析、鉴定方法,进而推动生物气溶胶探测、识别技术进步具有重要意义.
Three kinds of typical microorganisms, Bacillus subtilis, Aspergillus niger spores and Aspergillus niger were selected to prepare biological samples. The infrared reflectance spectra of three kinds of microorganisms in the range of 2.5-15μm were measured by microscopic infrared spectroscopy. The Krefel- KK), the birefringence of microbiological samples in the 6-14μm band was calculated.The water content of microbiological samples was calculated based on the infrared absorption spectra of water, protein and nucleic acid.Among the three kinds of microorganisms Based on the measured data of complex refractive index and water content in the far infrared band, the birefringence model of biological particles in the far infrared band of 6-14μm was constructed and the reliability of the model was analyzed.The rapid analysis of the biological samples for the construction of the model, Identification methods, thereby promoting bio-aerosol detection, identification of technological advances is of great significance.