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目的:检测Mre11基因及其蛋白在放疗后的大鼠腮腺和颌下腺组织中的表达变化。方法:选取近交系雄性Wistar大鼠60只,按单次照射0 Gy,3 Gy、6 Gy、9 Gy、12 Gy、15 Gy剂量分成6组,用60Coγ射线照射大鼠头颈部,2h后收集大鼠两侧腮腺和颌下腺组织。用透射电镜观察涎腺上皮细胞超微结构变化;用RT-PCR检测Mre11的基因表达情况;用免疫组化检测Mre11蛋白表达情况。结果:透射电镜显示:放射后大鼠腮腺腺泡细胞胞质和胞膜均出现不同程度的损坏,随着剂量的增加,这种损坏逐渐加剧,未见到再生、恢复的变化。颌下腺和导管变化不明显。RT-PCR检测Mre11mRNA表达无统计学差异。免疫组化检测Mre11蛋白表达有统计学差异。结论:治疗剂量的60Coγ照射对正常涎腺组织呈不可逆的损伤,损伤的程度具有剂量依赖性;大鼠涎腺细胞Mre11mRNA的表达无剂量依赖性;大鼠涎腺细胞Mre11蛋白的表达与辐射剂量以及组织类型有关,随剂量的增加先增强后减弱。
Objective: To detect the expression of Mre11 gene and its protein in the parotid and submandibular glands of rats after radiotherapy. Methods: Sixty male inbred male Wistar rats were divided into 6 groups according to the single dose of 0 Gy, 3 Gy, 6 Gy, 9 Gy, 12 Gy and 15 Gy. The rats were irradiated with 60Co γ ray for 2h After the rat parotid gland and submandibular gland tissue were collected. The ultrastructural changes of salivary gland epithelial cells were observed by transmission electron microscope. The gene expression of Mre11 was detected by RT-PCR. The expression of Mre11 protein was detected by immunohistochemistry. RESULTS: Transmission electron microscopy showed that the cytoplasm and membrane of rat parotid gland cells were damaged to varying degrees after radiation. With the increase of dose, the damage gradually increased, and no changes of regeneration and recovery were observed. Submandibular glands and ducts did not change significantly. RT-PCR detection of Mre11 mRNA expression was not statistically different. Immunohistochemical detection of Mre11 protein expression was statistically different. CONCLUSION: Irradiation with 60Coγ ray dose has an irreversible effect on the normal salivary glands, and the degree of injury is dose-dependent. The expression of Mre11 mRNA in rat salivary gland cells has no dose-dependent manner. The expression of Mre11 protein in rat salivary gland cells, Type, with the increase of dose increased first and then weakened.