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目的:探讨八珍汤对慢性难愈性创面大鼠创面肉芽组织增殖细胞核抗原(PCNA)与细胞凋亡的影响。方法:选择雄性SD大鼠,于背部制造全层皮肤缺损开放性创面。除正常创面对照组外,其余肌肉注射醋酸氢化可的松建立难愈性创面模型,随机分为模型组和八珍汤组。观察创面愈合时间;通过免疫组化结合图像分析等技术,观察各组大鼠创面肉芽组织PCNA表达情况;通过流式细胞技术,观察各组大鼠创面肉芽组织细胞凋亡情况。结果:模型组创面愈合时间较正常组明显延长(P<0.01),八珍汤组创面愈合时间比模型组明显缩短(P<0.01);模型组PCNA表达明显低于正常组(P<0.05),八珍汤组PCNA表达明显高于模型组(P<0.01),而明显低于正常组(P<0.01);模型组细胞凋亡明显高于正常组(P<0.01),八珍汤组细胞凋亡明显低于模型组(P<0.01),而明显高于正常组(P<0.01)。结论:八珍汤能明显促进慢性难愈性创面大鼠的创面愈合,其机制可能与促进创面肉芽组织细胞增殖,抑制创面肉芽组织细胞凋亡有关。
Objective: To investigate the effect of Bazhen decoction on proliferating cell nuclear antigen (PCNA) and apoptosis in granulation tissue of wounds with chronic refractory wounds. Methods: Male Sprague-Dawley rats were selected to create full-thickness skin defect open wounds on the back. In addition to the normal wound control group, the remaining intramuscular injection of hydrocortisone acetate establish a refractory wound model, were randomly divided into model group and Bazhen Decoction group. The healing time of wounds was observed. The expression of PCNA in wound granulation tissues was observed by immunohistochemistry and image analysis. The apoptosis of wound granulation tissue in each group was observed by flow cytometry. Results: The wound healing time of the model group was significantly longer than that of the normal group (P <0.01), and the wound healing time was significantly shorter in the Bazhen decoction group than that in the model group (P <0.01). The expression of PCNA in the model group was significantly lower than that in the normal group (P <0.05) , While the expression of PCNA in Bazhen decoction group was significantly higher than that in model group (P <0.01), but significantly lower than that in normal group (P <0.01). The apoptosis of model group was significantly higher than that in normal group (P <0.01) Apoptosis was significantly lower than the model group (P <0.01), but significantly higher than the normal group (P <0.01). Conclusion: Bazhen Decoction can significantly promote the healing of wounds in rats with chronic and refractory wounds. The mechanism may be related to promoting the proliferation of wound granulation tissue and inhibiting the apoptosis of wound granulation tissue.