激光共焦扫描显微镜大多采用点共焦扫描成像形式,扫描机构复杂,成本高。新型的线结构光共焦显微成像技术是对样品进行线共焦成像,只需对样品进行一维扫描就可以得到整个平面的像。设计了线结构光共焦成像实验装置,进行了线结构光共焦显微成像实验。实验结果证明了线结构光共焦成像的可行性,杂散光明显地减少,提高了成像清晰度,并且有光学层析能力。采用低照度高分辨率CCD代替价格昂贵的像增强器大大降低了系统成本。 Most confocal laser scanning microscopy point confocal scanning imaging, scanning mechanism is complex and costly. The new line-structured optical confocal microscopy is a line-confocal imaging of a sample. The entire planar image can be obtained by just one-dimensional scans of the sample. A line structure optical confocal imaging experimental apparatus was designed and the line structure optical confocal microscopy imaging experiment was carried out. The experimental results demonstrate the feasibility of confocal imaging of line-structured light with significantly reduced stray light, improved imaging clarity, and optical tomography capability. The use of low-light, high-resolution CCD instead of expensive image intensifier greatly reduces system costs.