论文部分内容阅读
本文初步探讨了6种抗血吸虫药物对血吸虫谷-丙转氨酶与谷-草转氨酶的影响,观察到将药物直接加至血吸虫匀浆中时,吐酒石2×10~(-4)克分子及南瓜子氨酸5×10~(-2)克分子的浓度皆能使谷-丙转氨酶的活力减低50％左右,后者尚可使谷-草转氨酶的活力增强24％.F30066的浓度为2×10~(-4)克分子时,可抑制谷-草转氨酶的活力28％,对谷-丙转氨酶则无明显影响.萱草根素、甲基敌百虫及玫苯胺对血吸虫匀浆的两种转氨酶均无明显作用.在体外培养试验中,吐酒石使血吸虫谷-丙转氨酶活力减低一半所需的药物浓度为直接加至匀浆中的5倍.血吸虫在含F30066 2×10~(-4)克分子的培基中培养4小时后,谷-丙转氨酶与谷-草转氨酶的活力分别下降25及14％.南瓜子氨酸、玫苯胺、甲基敌百虫及萱草根素对体外培养血吸虫的两种转氨酶活力均无明显影响.在体内试验中,除玫苯胺外,都能引起虫体明显退化,但两种转氨酶的活力似与对照组的相仿.根据试验结果,可以认为,有效药物的抗血吸虫作用可能不是在由转氨酶所促化的代谢环节上.
In this paper, the effects of six kinds of anti-schistosomiasis drugs on the serine aminotransferase and glutamic oxaloacetic transaminase of Schistosoma japonicum were preliminarily investigated. It was observed that when the drug was added directly to the homogenate of schistosome, 2 × 10 -4 mol / The concentration of 5 × 10 ~ (-2) mol·L-1 of cucurbitacin reduced the activity of glutamic-pyruvic transaminase by about 50%, while the latter increased the activity of glutamic-pyruvic transaminase by 24%. The concentration of F30066 was 2 × 10 ~ (-4) mol, the activity of glutamic oxaloacetic transaminase was inhibited by 28%, and there was no significant effect on glutamic-pyruvic transaminase.White grass carp, methyl trichlorfon, In transaminase had no significant effect.In vitro culture test, the spittoon to schistosome valley-aminotransferase activity reduced by half the required concentration of drug directly to the homogenate 5 times in. Schistosoma japonicum containing F30066 2 × 10 ~ ( -4), the viability of glutamic-pyruvic transaminase and glutamic-pyruvic transaminase decreased by 25 and 14%, respectively In vitro culture of schistosomiasis two aminotransferase activity had no significant effect in vivo tests, in addition to phenylaniline, can cause parasites Degradation, but the two transaminases vitality seems similar to the control group. Based on the test results, it is considered that the role of anti-schistosome drugs may not be effective in promoting the transaminase of the metabolic link.