论文部分内容阅读
目的:建立保健食品中牛磺酸含量的测定方法。方法:采用超高效液相色谱-蒸发光散射(UPLC-ELSD)法测定保健食品中牛磺酸含量。色谱条件:采用BEH Amide(50 mm×2.1 mm,1.7μm)色谱柱,梯度洗脱,流动相A为乙腈,流动相B为0.1%甲酸水,流速0.35m L·min~(-1),柱温:40℃,ELSD漂移管温度40℃,ELSD气体压力150 k Pa,增益值100。结果:牛磺酸浓度在0.10~2.0 mg·m L~(-1)范围内与峰面积呈良好线性关系(r=0.999 9),不同剂型保健食品中牛磺酸的回收率在98.5%~100.7%之间,RSD均小于1.3(n=6)。6批次液体制剂样品的测定结果分别为:38.60、8.702、0.501 0、5.152、5.085、3.821 mg·m L~(-1);3批次固体制剂样品的测定结果分别为:6.703、4.560和15.81 g/100 g;2批次软胶囊样品的测定结果分别为:24.20、8.206 g/100 g;1批硬胶囊样品的测定结果为19.18%。结论:本法经方法学验证,可用于保健食品中牛磺酸的测定。
Objective: To establish the method of determination of taurine in health foods. Methods: The content of taurine in health food was determined by UPLC-ELSD. Chromatographic conditions: gradient elution was carried out on a BEH Amide (50 mm × 2.1 mm, 1.7 μm) column with acetonitrile as mobile phase A, water 0.1% formic acid as mobile phase B, flow rate 0.35 m L · min -1, Column temperature: 40 ° C, ELSD drift tube temperature 40 ° C, ELSD gas pressure 150 kPa, gain 100. Results: There was a good linear relationship between the concentration of taurine and the peak area in the range of 0.10-2.0 mg · m L -1 (r = 0.999 9). The recovery rate of taurine in different formulations of health food was 98.5% 100.7%, RSD less than 1.3 (n = 6). The results of six batches of liquid preparations were 38.60,8.702,0.501,0,5.152,5.085,3.821 mg · m L -1, respectively. The results of three batches of solid preparations were 6.703, 4.560 and 15.81 g / 100 g. The results of two batches of soft capsule samples were 24.20 and 8.206 g / 100 g, respectively. The determination results for one batch of hard capsule samples were 19.18%. Conclusion: This method is validated by methodology and can be used for the determination of taurine in health foods.