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试验结果表明:以实生成苹果树上的芽作为外植体,培养在添加1—2mg/l 6-BA的SH或MS培养基上,在继代培养时芽的萌发率达92％以上,50天内芽数增殖5—6.8倍;同期内,液体旋转培养比琼脂静置培养芽条长度要增加3倍左右;芽条在0.2—0.4mg/lIBA中生根率80％以上;试管苗的移栽成活率85％以上。
The results showed that the shoots of apple trees were used as explants and cultured on SH or MS medium supplemented with 1-2 mg / l 6-BA. The germination rate of buds reached more than 92% in subculture, In the same period, the length of sprout buds increased by about 3 times than that of agar when cultured in the same period; the rooting rate of sprout buds was above 80% in 0.2-0.4 mg / l IBA; Planting survival rate above 85%.