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目的探讨乙型肝炎病毒(HBV)感染者血清中前S2抗原(PreS2)与乙肝病毒血清标志物,HBV-DNA之间的关系及临床意义。方法用酶联免疫吸附试验(ELISA)对乙肝病毒(HBV)血清标志物和乙肝病毒前S2抗原进行检测;用荧光定量PCR法检测HBV-DNA。结果在520例HBV-DNA阳性血清中,HBeAg阳性率为46.1%,前S2抗原阳性率为92.1%,两者相比较差异有统计学意义(P<0.01)。520例标本按照HBV-DNA含量高低分为四组,每组前S2抗原和HBeAg的阳性率进行比较,每组两者之间比较差异有统计学意义(P<0.01)。结论前S2抗原与HBeAg和HBV-DNA密切相关,前S2抗原较HBeAg更敏感反映体内乙肝病毒存在和复制状态,是乙肝病毒存在和复制较为直接的标志。
Objective To investigate the relationship between serum pre-S2 antigen (PreS2) and hepatitis B virus (HBV) serum markers and HBV-DNA in patients with hepatitis B virus (HBV) infection and its clinical significance. Methods Serum markers of hepatitis B virus (HBV) and pre-S2 antigen of hepatitis B virus were detected by enzyme-linked immunosorbent assay (ELISA). HBV-DNA was detected by real-time quantitative PCR. Results Among 520 HBV-DNA positive sera, the positive rate of HBeAg was 46.1% and the positive rate of pre-S2 antigen was 92.1%. There was significant difference between the two (P <0.01). 520 cases were divided into four groups according to the level of HBV-DNA, the positive rates of pre-S2 antigen and HBeAg in each group were compared, the difference between the two groups was statistically significant (P <0.01). Conclusions The pre-S2 antigen is closely related to HBeAg and HBV-DNA. The pre-S2 antigen is more sensitive than HBeAg to reflect the presence and replication of hepatitis B virus in vivo, which is a direct marker of the presence and replication of HBV.