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研究认为,穗花牡荆(Vitex agnus-castus)果实的乙醇提取物(Vitex)具有杀伤细胞的作用,可诱导数种人癌细胞凋亡。本次探讨了Vitex诱导细胞凋亡的机制。 方法:用Vitex处理人胃癌细胞(Kato III),用琼脂糖凝胶电泳法分析DNA片段化。用DTNB法检测处理细胞的GSH量。用Western印迹?
Studies have shown that the Vitex agnus-castus fruit extract (Vitex) has the effect of killing cells and can induce apoptosis in several human cancer cells. This study explored the mechanism of Vitex-induced apoptosis. METHODS: Human gastric cancer cells (Kato III) were treated with Vitex, and DNA fragmentation was analyzed by agarose gel electrophoresis. The amount of GSH in the treated cells was measured by the DTNB method. With Western blot?