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目的通过观察异丙肾上腺素和普萘洛尔分别对BALB/C小鼠腹腔巨噬细胞分泌肿瘤坏死因子(TNF-α)和白介素-10(IL-10)以及吞噬功能的影响,分析β肾上腺素能受体对巨噬细胞细胞功能的调节作用。方法将培养的BALB/C小鼠腹腔巨噬细胞细胞分为4组:空白组用生理盐水处理作为空白对照组;脂多糖组以10μg/ml脂多糖刺激;普萘洛尔组分别以不同浓度的普萘洛尔(0.1、1、10μmol/L)预处理后,再以10μg/ml脂多糖刺激;异丙肾上腺素组分别以不同浓度的异丙肾上腺素(10、100、300μmol/L)预处理后,再以10μg/ml LPS刺激。加入不同浓度药物培养6 h后,采用酶联免疫法测定各培养瓶上清液中TNF-α的浓度,24 h后测定IL-10的浓度,并用中性红吞噬试验检测巨噬细胞吞噬功能。结果在用脂多糖刺激的情况下,普萘洛尔呈浓度依赖性的增加TNF-α分泌,减少IL-10分泌,并使巨噬细胞吞噬功能下降,而异丙肾上腺素(ISO)作用却正好相反。结论对BALB/C小鼠,可以通过腹腔巨噬细胞膜上的β肾上腺素能受体,实现对炎症介质的释放和细胞吞噬能力的双向调节作用。
Objective To investigate the effects of isoproterenol and propranolol on the secretion of TNF-α and IL-10 and the phagocytic function of peritoneal macrophages in BALB / c mice. Regulatory effect of the hormone receptor on macrophage cell function. Methods BALB / C mice peritoneal macrophages were divided into 4 groups: blank group treated with normal saline as blank control group; lipopolysaccharide group stimulated by 10μg / ml lipopolysaccharide; propranolol group treated with different concentration Propranolol (0.1, 1, 10μmol / L), and then stimulated with 10μg / ml lipopolysaccharide. The isoproterenol groups were treated with different concentrations of isoproterenol (10,100,300μmol / L) After pretreatment, stimulation with LPS at 10 μg / ml was performed. After adding different concentrations of drug for 6 h, the concentration of TNF-α in each flask supernatant was measured by enzyme-linked immunosorbent assay. The concentration of IL-10 was measured 24 h later, and phagocytosis of macrophages was detected by neutral red phagocytosis . Results Propranolol increased the secretion of TNF-α, decreased the secretion of IL-10 and decreased the phagocytosis of macrophages in a dose-dependent manner under the stimulation of lipopolysaccharide, while the effect of isoproterenol (ISO) exactly the opposite. Conclusion BALB / C mice can regulate the release of inflammatory mediators and the ability of phagocytosis through β-adrenergic receptors on the membrane of peritoneal macrophages.