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目的:建立保健食品中脂肪酸的测定方法。方法:采用毛细管柱气相色谱技术,同时测定月桂酸、豆蔻酸、棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸、γ-亚麻酸、α-亚麻酸、花生酸、花生四烯酸、EPA、山俞酸、DPA、DHA,以正己烷为溶剂,色谱柱为FFAP毛细管柱(30 m×0.25 mm×0.25μm),检测器为FID。结果:各种脂肪酸色谱的分离度均大于1.5,平均回收率为89.9%~99.9%,相对标准偏差为0.07%~4.58%(n=6)。结论:本方法操作简便准确,适用于保健食品中多种脂肪酸的同时测定。
Objective: To establish a method for the determination of fatty acids in health foods. Methods: Capillary gas chromatography was used to determine the contents of lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, γ-linolenic acid, α-linolenic acid, Acid, EPA, behenic acid, DPA, DHA and n-hexane as solvent. The column was FFAP capillary column (30 m × 0.25 mm × 0.25 μm) and the detector was FID. Results: The separation of various fatty acids was more than 1.5. The average recoveries were 89.9% -99.9% with relative standard deviations of 0.07% -4.58% (n = 6). Conclusion: The method is simple and accurate. It is suitable for the simultaneous determination of various fatty acids in health foods.