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为了建立简便、快速筛选高效、低毒的新型抗氧化药物的方法,本研究采用快原子轰击离子化质谱(FAB-MS)对酚类化合物抗氧化作用强弱进行了研究。考察了基质、轰击时间和样本浓度对FAB-MS谱中分子离子(M+·)相对强度的影响,研究了黄酮类化合物在FAB-MS谱中的I(M+·)/I([M+H]+)比值与其抗氧化活性的相关性。对12种酚类化合物的抗氧化活性进行了预测与评价,并与硫代巴比妥酸法(TBA)检测的脂质过氧化抑制率进行比较,验证FAB-MS方法的可靠性。结果表明,由FAB-MS获得的I(M+·)/I([M+H]+)比值能较好地反映酚类化合物抗氧化作用的强弱,可以作为抗氧化剂的体外筛选或预测方法,有助于加速新型抗氧化药物的发现。
In order to establish a simple and rapid screening method for novel anti-oxidant drugs with high efficiency and low toxicity, the antioxidant activity of phenolic compounds was investigated by FAB-MS in this study. The effects of matrix, bombardment time and sample concentration on the relative intensities of molecular ions (M + ·) in FAB-MS spectra were investigated. I (M +) / I ([M + H ] +) Ratio and its antioxidant activity. The antioxidant activity of 12 phenolic compounds was predicted and evaluated, and compared with the lipid peroxidation inhibition rate detected by thiobarbituric acid (TBA) to verify the reliability of FAB-MS method. The results showed that the ratio of I (M +) / I ([M + H] +) obtained by FAB-MS could better reflect the antioxidant activity of phenolic compounds and could be used as an in vitro screening or prediction method of antioxidants , Helps to speed up the discovery of new anti-oxidants.