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目的:观察平消胶囊的抗肿瘤作用及其初步的作用机制。方法:提取平消胶囊活性部位,用于体外抗肿瘤细胞增殖和诱导肿瘤细胞凋亡;灌胃给予平消胶囊,采用4种人体肿瘤裸鼠移植瘤模型观察体内抑瘤作用及初步毒性反应。结果:平消胶囊提取物对10种人体肿瘤细胞具有一定的体外增殖抑制作用,体外作用72h,其IC50值在400-900μg/m L之间。平消胶囊连续经口灌胃给药14d,对人乳腺癌MDA-MB-231、人甲状腺癌SW579、人肠癌colo205、人胰腺癌PANC-1的最大抑瘤率分别达到59.00%、53.13%、49.51%、52.14%,且对动物体质量和脏器没有明显影响。平消胶囊提取物能诱导人乳腺癌细胞MDA-MB-231和人甲状腺癌细胞SW579凋亡。结论:平消胶囊对人乳腺癌、甲状腺癌、肠癌、胰腺癌的裸鼠移植瘤生长有明显的抑制作用,量效关系明显,且对主要脏器无明显影响,其机制之一为诱导肿瘤细胞的凋亡。
Objective: To observe the antitumor effect of Pingxiao capsule and its primary mechanism of action. Methods: The active site of Pingxiao capsule was extracted for anti-tumor cell proliferation in vitro and apoptosis of tumor cells was induced. The Pingxiao capsule was given intragastrically. Four kinds of human tumor xenografts were used to observe the antitumor activity and primary toxicity. Results: The extract of Pingxiao Capsule had certain in vitro proliferation inhibitory effect on 10 kinds of human tumor cells in vitro for 72h with IC50 of 400-900μg / mL. Pingxiao capsule sustained oral administration of 14d, the maximum inhibitory rate of human breast cancer MDA-MB-231, human thyroid cancer SW579, human colorectal cancer colo205, human pancreatic cancer PANC-1 were 59.00%, 53.13% , 49.51% and 52.14%, respectively, and had no significant effect on the body weight and organs of animals. Pingxiao capsule extract can induce human breast cancer cells MDA-MB-231 and human thyroid cancer cells SW579 apoptosis. Conclusion: Pingxiao capsule can significantly inhibit the growth of human breast cancer, thyroid cancer, colon cancer and pancreatic cancer xenografts in nude mice. The dose-effect relationship is obvious and there is no obvious effect on the major organs. One of the mechanisms is induction Tumor cell apoptosis.