论文部分内容阅读
为了寻找控制葡萄果粒形状的基因位点,本研究中对304个葡萄种质的果形性状了进行简化基因组关联分析。对葡萄种质材料的基因组DNA进行测序,平均测序深度为8.13×,共开发466 618个SLAF标签,其中多态性SLAF标签有392 374个。比对测序分析获得了4 241 729个SNP标记,进一步根据完整度大于0.8以及次要基因型频率大于0.05的标准进行过滤,共得到481 192个SNP。本次检测到的控制葡萄果粒形状的相关位点为首次报道,除基于一般线性模型的SNP rs26137624外,其余检测表型遗传变异贡献率均大于11%。基于一般线性模型和混合线性模型分别关联到7个和3个显著性SNP标记,且均位于2号染色体上。通过筛选每个显著性SNP标记上下游区域,对关联区域进行功能基因挖掘,共得到10个有预测功能注释的基因和7个未知功能的基因。
In order to search for the genetic loci controlling the shape of grape fruit, the genotypes of 304 grape germplasms were analyzed by simplified genomic association. Genomic DNA from grape germplasm was sequenced. The average depth of sequencing was 8.13 ×. A total of 466 618 SLAF markers were identified, of which 392 374 were polymorphic SLAF markers. A total of 4 241 729 SNP markers were obtained from the sequence analysis and further filtered according to the criteria that the completeness was greater than 0.8 and the minor genotype frequency was greater than 0.05. A total of 481 192 SNPs were obtained. For the first time, the related sites controlling the shape of grape fruit were detected. The contribution rate of other detected phenotypic variations was more than 11% except for the SNP rs26137624 based on the general linear model. Based on the general linear model and the mixed linear model, 7 and 3 significant SNP markers were linked, respectively, and were located on chromosome 2. By screening the upstream and downstream regions of each significant SNP marker, functional gene mining was performed on the related regions. Ten genes with predicted function annotation and seven genes with unknown function were obtained.