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目的:筛选透骨草抑制肺癌A549细胞增殖的有效部位,为透骨草药效物质基础研究阐明并提供依据。方法:将透骨草分别用石油醚、乙酸乙酯、正丁醇萃取,萃取后各部位分别在体外用MTT法检测透骨草对肺癌A549细胞的增殖影响,并根据实验结果确定透骨草的有效部位。结果:透骨草的各部位均有抑制肺癌A549细胞增长的作用,其中乙酸乙酯层提取物抑制肺癌A549细胞增殖的作用最为明显;而石油醚提取物的抑制作用最弱。结论:透骨草具有确切的抑制肺癌A549细胞增殖的作用,其乙酸乙酯层提取物为其有效成分集中部位。
OBJECTIVE: To screen the effective sites of the inhibitory effect of Speranskia spp. On the proliferation of lung cancer A549 cells and to clarify and provide the basis for the basic research of the medicinal substances of Speranskia. Methods: The extracts were extracted with petroleum ether, ethyl acetate and n-butanol, respectively. After extraction, the proliferation of A549 cells was determined by MTT assay in vitro. The effective site. Results: All parts of the extract of honeysuckle inhibited the growth of lung cancer A549 cells. Among them, ethyl acetate extract inhibited the proliferation of lung cancer A549 cells most obviously, while the petroleum ether extract had the weakest inhibitory effect. Conclusion: Viagra has the exact effect of inhibiting the proliferation of lung cancer A549 cells, and its ethyl acetate extract is the effective component of its concentration.