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目的 :观察大鼠 BERH - 2肝癌细胞与激活 B细胞的细胞融合大鼠肝癌疫苗对肿瘤的预防和治疗作用。方法 :应用 PEG将大鼠 BERH- 2肝癌细胞与激活 B细胞融合 ,制备细胞融合大鼠肝癌疫苗 ;部分肝癌疫苗免疫动物前经 6 0 Co照射 ;大鼠被肝癌疫苗免疫之前或之后 ,接种肝癌 BERH- 2细胞或组织块 ,观察疫苗对肝癌的预防和治疗作用。结果 :经细胞融合疫苗保护的大鼠可获 10 0 %无瘤生存 (8/8) ,而 HERH- 2或照射后 BERH- 2对照组大鼠均生长肿瘤死亡 (0 /8)。经皮下接种细胞融合肝癌疫苗治疗大鼠 ,肝内注射肝癌细胞的大鼠可获 85 %以上无瘤存活。照射后细胞融合肝癌疫苗与未照射的获得同样治疗效果。不经筛选的细胞融合疫苗也获相同治疗效果 ,而对照组大鼠均生长肿瘤后死亡。结论 :细胞融合肝癌疫苗对大鼠有预防和治疗作用 ,照射的细胞融合大鼠肝癌疫苗和不经筛选的融合细胞同样具有较好的治疗作用。
OBJECTIVE : To observe the prevention and treatment of rat hepatocellular carcinoma vaccine against hepatocellular carcinoma cells with BERH-2 liver cancer cells and activated B cells. METHODS: PEG was used to fuse rat BERH-2 hepatoma cells with activated B cells to prepare cell fusion rat hepatocellular carcinoma vaccine; partial liver cancer vaccine-immunized animals were irradiated with 60 Co; and rats were immunized with liver cancer before or after being immunized with liver cancer vaccines. BERH-2 cells or tissue blocks were observed for the prevention and treatment of liver cancer. RESULTS: Rats protected with cell fusion vaccine had 100% tumor-free survival (8/8), while rats that died of HERH-2 or BERH-2 control group all had tumor death (0/8). After subcutaneous inoculation of cell fusion liver cancer vaccine in rats, hepatic injection of hepatoma cells in rats can be more than 85% of tumor-free survival. After irradiation, cell fusion liver cancer vaccine and non-irradiation have the same therapeutic effect. The unselected cell fusion vaccine also achieved the same therapeutic effect, while the control group died after the tumor growth. Conclusion : The cell fusion liver cancer vaccine has preventive and therapeutic effects on rats. Irradiated cell fusion rat liver cancer vaccine and non-screened fusion cells also have good therapeutic effects.