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目的:探讨金鸡菊提取物体外抗氧化活性。方法:采用分光光度法,测定金鸡菊提取物对羟基(OH)自由基、超氧阴离子(O 2-.)自由基及1,1-二苯基-2-苦肼基自由基(DPPH)的清除能力,通过多元线性回归分析,考察金鸡菊提取物含量及其体外抗氧化活性的相关性,并同维生素(VC)进行了比较。结果:金鸡菊提取物对.OH自由基,O 2-.自由基,DPPH自由基均有较好的清除作用,在供试质量浓度范围内(0.2～1.0 g.L-1)对各自由基的清除效率与金鸡菊提取物浓度有一定量效关系,当质量浓度达1.0 g.L-1,其中50%乙醇洗脱物对OH自由基、O 2-.自由基和DPPH自由基的清除率可达86.19%,97.90%和71.85%,且清除能力与Vc相当。结论:金鸡菊提取物具有显著的体外抗氧化作用。
Objective: To investigate the anti-oxidative activity of extract of Coreopsis chinensis in vitro. Methods: The contents of hydroxyl radical (OH), superoxide anion (O2-) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) were determined by spectrophotometry. The multivariate linear regression analysis was used to examine the correlation between the content of extract of Coreopsis chinense and the anti-oxidative activity in vitro and with the vitamin (VC). Results: The extract of Coreopsis chinense had good scavenging activities on the free radical of OH, O 2- and DPPH free radicals. The free radicals of each of them were tested in the concentration range of 0.2 ~ 1.0 gL -1 The efficiency of eradication had a dose-effect relationship with the concentration of extract of Coreopsis chinensis. When the mass concentration was 1.0 gL-1, the elution rate of 50% ethanol eluate on OH, O2- and DPPH free radicals was 86.19 %, 97.90% and 71.85%, respectively, and the scavenging capacity is equivalent to Vc. Conclusion: The extract of Coreopsis chinense has significant antioxidant activity in vitro.