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应用多聚酶链式反应 (PCR)技术检测结果表明 ,海南槟榔黄化病株及台湾槟榔黄化病株样品用椰子致死性黄化病病原特异性扩增引物对P1、K2 3- 1及Phytoplasma通用引物对Nested ,Fus - 0 7进行扩增 ,均未发现有特异的PhytoplasmaDNA谱带 ,呈阴性结果。其他 3个感染Phytoplasma的植物对照样品用引物对Nested、Fus - 0 7扩增 ,均得到特异的Phyto plasmaDNA片断谱带 ;引物对P1、K2 3- 1扩增只有 1S2 8- 3( 2 )有特异的PhytoplasmaDNA片断谱带 ,其他样品均呈阴性。试验结果说明 ,海南及台湾的槟榔黄化病与椰子致死黄化病的病原之间的亲缘关系比较疏远 ,海南及台湾的槟榔黄化病发生不是Phytoplasma引起
The results of polymerase chain reaction (PCR) showed that the samples of betel yellowing of betel yellow in Hainan and the yellowing of betel yellow in Taiwan were common to P1, K2-3 and Phytoplasma by pathogen-specific amplification primers Primers for Nested, Fus - 0 7 amplification, no specific Phytoplasma DNA bands were found, negative results. The other three Phytoplasma-infected plant samples were amplified with primers Nested and Fus-0 7, and all of them had a specific Phyto-DNA fragment. The primer pairs P1 and K2 were only 1S2-8-3 Specific Phytoplasma DNA fragment bands, other samples were negative. The test results show that the genetic relationship between betel yellowing disease in Hainan and Taiwan is far from the pathogen of coconut yellowing disease, and the incidence of betel yellowing disease in Hainan and Taiwan is not caused by Phytoplasma