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制备一种新型速效胰岛素类似物PInsulin(PIns),利用Autoflex ToF/ToF质谱法对其进行鉴定,采取福林-酚试剂法测定其浓度;以人胰岛素、赖脯胰岛素作为对照品肌肉注射测定其对雄性SD大鼠的降血糖作用,并通过分子筛层析法考察其自聚合性质。质谱法测得PIns的Mr为6286.50,浓度为0.227mg/ml;动物实验表明注射PIns后,大鼠血糖降至最低基础血糖浓度百分数和恢复至基础血糖浓度所需时间均显著小于人胰岛素(P<0.01);注射PIns后基础血糖浓度百分数的最低值与人胰岛素相当;分子筛层析法结果显示PIns的滞留时间大于人胰岛素,表明PIns自身聚合能力低于人胰岛素,是一种具有良好发展前景的新型速效胰岛素类似物。
A new type of fast-acting insulin analog PInsulin (PIns) was prepared and identified by Autoflex ToF / ToF mass spectrometry. The concentration of PInsulin (PIns) was determined by Fulin-phenol reagent method. The human insulin and insulin lispro Hypoglycemic effect on male SD rats and their self-polymerization properties were investigated by molecular sieve chromatography. The result of mass spectrometry showed that the Mr of PIns was 6286.50 and the concentration was 0.227mg / ml. The animal experiments showed that after PIns injection, the basal blood glucose concentration and the time needed to recover to the basal blood glucose concentration were all significantly lower than those of human insulin (P <0.01). The lowest percentage of basal blood glucose concentration after injection of PIns was comparable to that of human insulin. The results of molecular sieve chromatography showed that the retention time of PIns was longer than that of human insulin, indicating that PIns had lower self-polymerization ability than human insulin Of the new rapid-acting insulin analogues.