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为探讨肺癌患者体内肿瘤坏死因子 (TNF)与自然杀伤 (NK)细胞活性水平及影响二者活性的临床因素。采用外周血诱生TNF测定法检测TNF活性 ;应用荧光检测法分析细胞毒性效应DNA以观察NK细胞活性。结果发现肺癌患者NK细胞活性显著低于正常人和非癌胸病病人 ,并随病期进展、肿瘤增大及淋巴结转移而加重 ,不受病理学类型影响。TNF活性水平显著高于非癌胸病人与正常人 ,而与病期、肿瘤大小、淋巴结转移与否及病理学类型无关。结果认为肺癌病人体内细胞免疫功能处于严重失衡状态 ,表现为NK细胞活性下降 ,全血诱生TNF活性呈高水平状态。检测二者活性可为肺癌病人的综合治疗提供理论依据。
To investigate the level of activity of tumor necrosis factor (TNF) and natural killer (NK) cells in patients with lung cancer and the clinical factors affecting their activity. The TNF activity was measured by peripheral blood-induced TNF assay; cytotoxic effect DNA was analyzed by fluorescence detection to observe NK cell activity. The results showed that the activity of NK cells in lung cancer patients was significantly lower than that of normal and non-cancer breast disease patients, and aggravated with the progression of the disease, tumor enlargement, and lymph node metastasis, and was not affected by the pathological type. The level of TNF activity was significantly higher than that of non-cancerous chest and normal subjects, but not with the stage of disease, tumor size, lymph node metastasis or pathological type. The results suggest that the cellular immune function in patients with lung cancer is in a severely imbalanced state, showing a decrease in the activity of NK cells and a high level of TNF-inducing activity in whole blood. Detecting the activity of both can provide a theoretical basis for the comprehensive treatment of lung cancer patients.