目的　了解急性髓性白血病M5亚型 (AML -M5 )患者T细胞受体重排删除DNA环 (TRECs)的含量和TCRVβ基因谱系利用和克隆性 ,从而了解AML患者的胸腺近期输出功能和TCRVβ亚家族T细胞增殖特点 .方法　利用实时定量PCR(TaqMan)方法检测 5例M5患者外周血单个核细胞TRECs的水平 ,并根据外周血中CD3阳性率计算CD3细胞中TRECs水平 .利用RT -PCR和基因扫描分析患者外周血单个核细胞的TCRVβ2 4个亚家族基因表达和克隆性 .9例正常人外周血作为对照 .结果　M5患者外周血中TRECs含量为 0 .76± 1.2 1/ 10 0 0CD3+ 细胞 ,明显低于正常人TRECs水平 (6 .84± 4 .71/ 10 0 0CD3+ 细胞 ,p <0 .0 5 ) .5例患者外周血T细胞表达不同数量Vβ亚家族 (2 - 16个 ) .基因扫描分析显示 4例病人外周血中的一些Vβ亚家族出现克隆性T细胞 ,Vβ1,Vβ15和Vβ2 1克隆性T细胞均分别见于 3例病人中 .结论　率先报道了AML -M5型患者胸腺近期输出naiveT细胞功能明显降低 ,尽管整体T细胞免疫功能低下 ,患者仍存在优势利用和克隆性增殖Vβ亚家族T细胞 ,提示其具有一定地对白血病细胞相关抗原产生特异性免疫反应的能力 Objective To understand the content of T cell receptor rearrangement deletion loop (TRECs) and the utilization and cloning of TCRVβ gene in patients with acute myeloid leukemia M5 subtype (AML-M5) so as to understand the recent thymus output function and TCRVβ subtypes Family T cell proliferation.Methods The levels of TRECs in peripheral blood mononuclear cells from 5 M5 patients were detected by real-time quantitative PCR (TaqMan), and the level of TRECs in CD3 cells was calculated according to the positive rate of CD3 in peripheral blood.Using RT-PCR and gene The TCRVβ2 4 subfamily gene expression and clonality in peripheral blood mononuclear cells of patients were analyzed by scanning.Results The peripheral blood of M5 patients had TRECs content of 0.76 ± 1.2 1 / 100CD3 + (6. 84 ± 4 .71 / 100 CD3 + cells, p <0.05) .There were 2 to 16 Vβ subfamilies (2 to 16) in peripheral blood T cells of 5 patients, Scanning analysis showed that some Vβ subfamilies in peripheral blood of 4 patients showed clonal T cells, and Vβ1, Vβ15 and Vβ2 1 clonal T cells were found in 3 patients respectively.Conclusion The first report of recent thymus output of AML-M5 patients naive T cells are significantly reduced in function, despite the overall T-cell immune dysfunction, there are still advantages in the use and clonal proliferation of V beta subfamily T cells, suggesting that it has certain ability to produce specific immune response to leukemia-associated antigen