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目的:探讨大豆异黄酮(isoflovone,IS)对去卵巢大鼠空间学习记忆和脑内单胺类递质的影响。方法:选用青年SD雌性大鼠,去除双侧卵巢造模。随机分为6组,对照组、模型组、苯甲酸雌二醇组、大豆异黄酮低、中、高剂量组。苯甲酸雌二醇组给大鼠苯甲酸雌二醇ip 0.2 mg·kg-1每周1次,IS低、中、高剂量组剂量分别ig给以大豆异黄酮30,60,120 mg·kg-1·d-1,共3个月。观察各组大鼠Morris水迷宫学习能力,高效液相检测海马内单胺类神经递质,放射免疫法测定下丘脑内促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)的含量。结果:①与对照组比较,模型组前3 d定位航行学习能力和空间探索能力明显降低(P<0.05);与模型组比较苯甲酸雌二醇组、IS中剂量组前3 d定位航行学习能力和空间探索能力明显提高(P<0.05)。②与对照组比较模型组海马去甲肾上腺素(norepinephrine,NE),多巴胺(dopamine,DA),5-羟色胺(5-hydroxytryptamine,5-HT)含量明显降低(P<0.05);与模型组比较IS高剂量组海马NE含量增高(P<0.05);与模型组比较中剂量组和苯甲酸雌二醇组海马DA和5-HT含量明显增高(P<0.05)③与对照组比较模型组GnRH含量明显增高(P<0.05);与模型组比较苯甲酸雌二醇组、IS中、高剂量组GnRH含量明显降低(P<0.05)。结论:中剂量大豆异黄酮通过提高海马内NE,DA,5-HT含量,降低海马GnRH含量,提高去卵巢大鼠空间学习记忆能力。
Objective: To investigate the effect of isofloxone (IS) on spatial learning and memory and monoamine neurotransmitters in the brain of ovariectomized rats. Methods: Female SD rats were selected to remove bilateral ovaries. Randomly divided into 6 groups, control group, model group, estradiol benzoate group, soy isoflavones low, medium and high dose group. Rats in estradiol benzoate group were given estradiol benzoate ip 0.2 mg · kg-1 once a week, while those in IS, middle and high dose groups were treated with 30, 60, 120 mg · kg-1 of soy isoflavones · D-1, a total of 3 months. The learning ability of Morris water maze, the monoamine neurotransmitter in hippocampus were detected by HPLC, and the content of gonadotropin-releasing hormone (GnRH) in hypothalamus was determined by radioimmunoassay. Results: ① Compared with the control group, the navigational ability and space exploration capability of the model group decreased significantly in the first 3 days (P <0.05). Compared with the model group, the estradiol benzoate group and IS medium dose group Ability and ability to explore space increased significantly (P <0.05). Compared with the control group, the content of norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) in the hippocampus in model group was significantly decreased (P <0.05) (P <0.05). Compared with the model group, the content of DA and 5-HT in hippocampus of middle dose group and estradiol benzoate group were significantly increased (P <0.05). Compared with the control group, GnRH (P <0.05). Compared with the model group, GnRH level in the estradiol benzoate group and IS medium and high dose groups was significantly decreased (P <0.05). Conclusion: Middle dose of soy isoflavones can improve spatial learning and memory in ovariectomized rats by increasing NE, DA and 5-HT contents in hippocampus, decreasing hippocampal GnRH content.