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目的 研究钙结合素 D2 8k(Ca BP)在帕金森病 (PD)发病机制中的作用 .方法 给 C5 7BL小鼠 ip 1-甲基 - 4-苯基 -1,2 ,3,6 -四氢吡啶 (MPTP) ,30 mg· kg- 1 MPTP每 2 4h注射 1次 ,连续应用 3d以建立 PD鼠模型 .用原位杂交法检测Ca BP m RNA在 PD鼠和对照鼠黑质神经元中的表达变化 .结果 Ca BP m RNA阳性神经元主要分布于黑质致密部(SNC) .在 MPTP处理鼠的 SNC神经元 ,Ca BP m RNA的表达水平明显降低 .模型鼠和对照鼠的 SNC中 Ca BP m RNA阳性神经元的数量有差异 (144± 15 ) vs (88± 10 ) ,(P <0 .0 5 ) .与对照相比 ,MPTP处理组小鼠 SNC中表达 Ca BPm RNA神经元的杂交信号强度也有降低 .结论 Ca BP可能通过对黑质多巴胺能神经元的保护作用来抵御 PD的发生
Objective To study the role of calcineurin D2 8k (Ca BP) in the pathogenesis of Parkinson’s disease (PD) .Methods The C5 7BL mouse ip 1-methyl-4-phenyl-1,2,6,6 Hydrogenpyridine (MPTP) and MPTP 30 mg · kg-1 once every 24 hours were used to establish the model of PD mice.An in situ hybridization method was used to detect the expression of CaBP m RNA in substantia nigra neurons of PD mice and control mice .Results Ca BP m RNA positive neurons were mainly distributed in substantia nigra pars compacta (SNC) .In MPTP-treated SNC neurons, the expression level of Ca BP m RNA was significantly decreased in SNC of model and control mice The number of Ca BP m RNA-positive neurons in CaTP m RNA-treated group was (144 ± 15) vs (88 ± 10), (P <0.05) Of the hybridization signal intensity also decreased.Conclusion Ca BP may protect against the occurrence of PD by protecting the substantia nigra dopaminergic neurons