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目的:研究蒙药五根油剂的抗衰老作用。方法:将60只昆明小鼠(雌雄各半)随机分为5组,即正常组、模型组、蒙药五根油剂低剂量组和高剂量组、维生素E组,除正常对照组外,其余各组均采用颈背部皮下注射D-半乳糖,150 mg/(kg·d),建立亚急性衰老模型,同时分别灌胃给药蒙药五根油剂低剂量[0.8 g/(kg·d)]、高剂量[3.2 g/(kg·d)]和维生素E[0.86 mg/(kg·d)]。连续给药7周后处死动物,测定血清和脑组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)及丙二醛(MDA)含量变化;检测脾脏和胸腺指数变化。结果:蒙药五根油剂各给药组可显著提高衰老小鼠血清及脑组织中的SOD、GSH-px含量,降低MDA含量;降低脾脏指数和胸腺指数,其中高剂量组的作用优于低剂量组。结论:蒙药五根油剂可明显提高衰老小鼠抗氧化能力,具有抗衰老作用。
Objective: To study the anti-aging effect of Mongolian medicine. Methods: Sixty Kunming mice (male and female mice) were randomly divided into five groups: normal group, model group, Mongolian medicine five oil low dose group and high dose group, vitamin E group, except the normal control group, the rest Subcutaneous injection of D-galactose 150 mg / (kg · d) on the back of the neck was used to establish the sub-acute aging model. At the same time, five doses of Mongolian medicine (0.8 g / (kg · d) ], High dose [3.2 g / (kg · d)] and vitamin E [0.86 mg / (kg · d)]. The rats were sacrificed 7 weeks after continuous administration, and the content of superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and malondialdehyde (MDA) in serum and brain tissue were determined. And thymus index changes. Results: The Mongolian medicine five oil treatment groups can significantly increase the content of SOD, GSH-px in the serum and brain tissue of aging mice, reduce the content of MDA, reduce the spleen index and thymus index, and the effect of high dose group is better than low Dose group. Conclusion: Mongolian medicine five oil can significantly improve the antioxidant capacity of aging mice, with anti-aging effect.