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目的观察睡眠剥夺应激对大鼠海马的损伤作用,并研究其对大鼠探究行为的影响。方法以改良多平台水环境(MMPM)建立睡眠剥夺模型,设大平台对照组和睡眠剥夺组。分别记录各组大鼠体质量、血浆糖皮质激素(GC)和神经元特异性烯醇化酶(NSE)水平以及乳酸脱氢酶(LDH)活性的变化;常规HE染色法观察海马损伤情况;旷场实验确定大鼠自主探究能力。结果睡眠剥夺5 d和7 d后大鼠血浆GC含量和LDH活性均显著高于对照组。睡眠剥夺3 d后血浆中NSE水平明显高于对照组,并有随时间延长而逐渐升高的趋势。病理染色和旷场实验显示,睡眠剥夺3 d后大鼠即出现海马损伤和探究行为评分降低。结论睡眠剥夺对大鼠海马具有明显的损伤作用,可能是睡眠剥夺大鼠自主探究行为下降的重要原因。
Objective To observe the injury effect of sleep deprivation stress on hippocampus of rats and to study its effect on exploring behavior in rats. Methods A sleep deprivation model was established by improving multi-platform water environment (MMPM). A large platform control group and a sleep deprivation group were set up. The body weight, plasma levels of glucocorticoid (GC) and neuron-specific enolase (NSE) and the activity of lactate dehydrogenase (LDH) in each group were recorded respectively. The damage of hippocampus was observed by routine HE staining. Field experiments to determine the ability of rats to explore spontaneously. Results After 5 days and 7 days of sleep deprivation, the contents of GC and LDH in plasma of rats were significantly higher than those of the control group. After 3 days of sleep deprivation, plasma NSE level was significantly higher than that of the control group, and gradually increased with time. Pathological staining and open-field experiments showed that rats in 3 days after sleep deprivation showed hippocampal damage and lowered exploration behavior scores. Conclusion Sleep deprivation can significantly damage the hippocampus of rats and may be an important reason for the spontaneous exploration of sleep deprivation rats.