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利用花粉管通道技术,将Bt基因导入大豆品种。对132株D1代植株进行PCR检测,得到5株阳性转化植株。再将获得的5株D1代阳性转化植株的种子放在温箱中发芽,提取DNA进行检测,结果得到2株D2代稳定遗传的阳性转化植株。用X-Glue溶液对转Bt基因132株D1代植株进行检测,结果没有发现阳性反应。另外,本实验还采用荧光制片方法从植物组织结构的角度证明利用花粉管通道方法导入外源基因的可行性,并提出花粉管通道方法操作的最佳时间为授粉后6～20h。
Using the pollen tube channel technology, the Bt gene was introduced into soybean varieties. 132 strains of D1 plants were tested by PCR and 5 positive transgenic plants were obtained. Then the seeds of the 5 positive plants of D1 generation obtained were germinated in a thermostat and the DNA was extracted for detection. Two positive transgenic plants stably inherited from D2 were obtained. X-Glue solution of transgenic Bt 132 strains of D1 plants were detected, the results did not find a positive reaction. In addition, this experiment also used fluorescence method to prove the feasibility of introducing foreign genes by pollen tube pathway from the perspective of plant tissue structure. The optimal time for pollen tube pathway was 6 ~ 20h after pollination.