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目的建立裂头蚴感染小鼠模型。方法分别采用曼氏迭宫绦虫裂头蚴头部灌胃法和全虫喂饲法建立裂头蚴病小鼠模型,比较两种方法的感染率与裂头蚴生长情况;采用连续转种的方式观察灌胃法建立动物模型的稳定性。结果采用灌胃法和全虫喂饲法建立裂头蚴病小鼠模型,感染率分别为93.00%和91.00%,差异无统计学意义(χ2=0.272,P>0.05);感染后2个月,两组剖检回收的裂头蚴长度分别为(5.13±1.71)cm和(5.56±1.26)cm,差异无统计学意义(t=-0.751,P>0.05);感染2个月灌胃组裂头蚴长度平均增加(5.09±0.58)cm,喂饲法增加(2.37±0.937)cm,差异有统计学意义(t=27.034,P<0.01)。每隔2个月对同一批裂头蚴应用灌胃法转种1次,连续转种18次后裂头蚴的感染性和活力无明显改变,每次转种时均可获取除头部外的新鲜虫体。结论用裂头蚴头部进行灌胃可建立稳定的裂头蚴感染小鼠模型。
Objective To establish a model of mouse infected with sporozoite. Methods The mice models of sparganosis were established by the method of head gavage and full feeding with Metaphysicum mansoni, respectively. The infection rates and the growth of sparganosis were compared between the two methods. The method of continuous transplanting was used to establish the method of gavage. Animal model stability. Results The mouse models of sparganosis were established by gavage and all-insect feeding method. The infection rates were 93.00% and 91.00%, respectively, with no significant difference (χ2 = 0.272, P> 0.05). Two months after infection, The length of the spleniccosis larvae recovered in group 2 was (5.13 ± 1.71) cm and (5.56 ± 1.26) cm, respectively, with no significant difference (t = -0.751, P> 0.05) (5.09 ± 0.58) cm, and the feeding method increased (2.37 ± 0.937) cm, the difference was statistically significant (t = 27.034, P <0.01). Every 2 months, the same batch of spore-larvae were treated by gavage and transplanted one time. After continuous transplanting for 18 times, the infection and viability of the sporozoite did not change significantly, and fresh worms . Conclusion The model of stably infected mouse with sparganosis can be established by gavage with the head of the spleens.