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斑茅(Saccharum arundinaceum Retz.)的生物产量高,对土壤条件要求低,可作为纤维素乙醇生产的原料作物在我国南方地区广泛种植。实验以斑茅为原料,采用液氨预处理法克服其水解顽抗性,并添加纤维素酶进行酶解,运用高效液相色谱(HPLC)测定了酶解液中的单糖含量。实验结果表明在纤维素酶添加量为15 FPU/(g当量葡聚糖)、预处理原料含水率为80%、预处理温度为130℃、预处理驻留时间为10 min、液氨与生物质的质量比例为2∶1时,葡聚糖和木聚糖的总转化率分别为69.34%和82.60%,相比于未作预处理的原料分别提高了573%和1 056%,单糖产量提高8倍。实验结果表明液氨预处理对斑茅是一种有效的预处理方式,并优于稀酸或湿爆法预处理,与酸预处理和氨爆法(AFEX)处理效果接近。
Saccharum arundinaceum Retz. Has high biological yield and low requirement for soil conditions. It can be widely used as a raw material for the production of cellulosic ethanol in southern China. Experiments were carried out using the Pterocephalus yunnanensis as raw material, and the pretreatment of liquid ammonia was used to overcome the tenacity of hydrolysis. The cellulase was added for enzymolysis. The content of monosaccharide in the hydrolyzate was determined by high performance liquid chromatography (HPLC). The experimental results showed that when the amount of cellulase added was 15 FPU / g, the moisture content of the pretreated raw material was 80%, the pretreatment temperature was 130 ℃, the pretreatment residence time was 10 min, The total mass conversion of dextran and xylan was 69.34% and 82.60%, respectively, when the ratio of mass to mass ratio was 2:1, which increased by 573% and 1056% respectively compared with those without pretreatment. Output increased by 8 times. The experimental results show that liquid ammonia pretreatment is an effective pretreatment method for M. diterpennis, which is superior to the pretreatment of dilute acid or wet explosion, and is close to the effect of acid pretreatment and ammonia explosion method (AFEX).