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目的:建立高效液相色谱法测定中药新药茱萸和胃止痛胶囊中吴茱萸碱和吴茱萸次碱的含量。方法:色谱柱为Ec lipse XDB-C18(4.6 mm×150 mm,5μm),流动相为乙腈-水(45∶55),柱温30℃,检测波长为250 nm。结果:吴茱萸碱和吴茱萸次碱的线性范围分别为0.051 0~0.560,0.050 8~0.559μg;平均回收率分别为97.5%(RSD 1.0%),98.7%(RSD1.5%)。结论:该法专属性强,准确稳定,重复现性好,可作为茱萸和胃止痛胶囊的质量控制方法。
Objective: To establish a HPLC method for the determination of evodiamine and rutaecarpine in Zhuxu and Weizhi Tong capsules. Methods: The column was Ec lipse XDB-C18 (4.6 mm × 150 mm, 5 μm). The mobile phase was acetonitrile-water (45:55). The column temperature was 30 ℃ and the detection wavelength was 250 nm. Results: The linear ranges of evodiamine and rutaecarpine were 0.051 0-0.560 and 0.050 8-0.559 μg respectively. The average recoveries were 97.5% (RSD 1.0%) and 98.7% (RSD 1.5%), respectively. Conclusion: This method is highly specific, accurate and stable, reproducible, and can be used as a quality control method for Fructus Corni and Weizhi Zhitong capsule.