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小麦面粉黄色度b*值是反映面粉颜色的重要指标,主要与籽粒黄色素含量有关。利用122对SSR引物、4对贮藏蛋白STS引物和10对AFLP引物组合,分析了中优9507×CA9632的71个DH系,构建了由173个位点组成的遗传连锁图,在小麦21个连锁群上覆盖2 881 cM。将该群体种植2年共计5个地点,测定籽粒黄色素和面粉黄色度b*值含量。采用复合区间作图法(CIM)进行了籽粒黄色素含量和面粉黄色度QTL分析。结果表明,面粉黄色度b*值的QTL位于染色体1DS、2DL、3A、4D、5D、6AL、6D和7AL上,其中7AL的QTL效应最大,贡献率为12.9%~37.6%;籽粒黄色素含量的QTL位于染色体2DL、3DL、4A、5A和7AL,其中7AL的QTL效应最大,贡献率为12.1%~33.9%。面粉黄色度b*值与籽粒黄色素含量共同的QTL位于7AL,与Xgwm264b紧密连锁,遗传距离分别为0~3.9 cM和0~0.9 cM。
Wheat flour yellow degree b * value is an important indicator of flour color, mainly related to the yellow pigment content of grain. Using 122 pairs of SSR primers, 4 pairs of storage protein STS primers and 10 pairs of AFLP primer combinations, 71 DH lines of Zhongyou 9507 × CA9632 were analyzed and a genetic linkage map of 173 loci was constructed. Twenty-one linkage maps of wheat The population covered 2,881 cM. The population was planted for two years a total of five locations, determination of yellow and yellow grain flour b * value of the content. QTL analysis of yellow pigment content and yellowness of grain was performed by composite interval mapping (CIM). The results showed that the QTL for the yellowness b * value of flour was located on the chromosomes 1DS, 2DL, 3A, 4D, 5D, 6AL, 6D and 7AL. The QTL effect of 7AL was the largest with the contribution rate of 12.9% -37.6% QTLs were located on chromosomes 2DL, 3DL, 4A, 5A and 7AL, of which 7AL had the largest QTL effect with the contribution rate of 12.1% -33.9%. The QTL for yellowness b * value and yellow pigment content of flour were located at 7AL, closely linked to Xgwm264b with genetic distances of 0-3.9 cM and 0-0.9 cM, respectively.