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由(Xanthomonas campestris pv.glycines)所致大豆斑疹病病斑隆起成疱。盆栽大豆感病品种(Chip-pewa和Horosoy)和它们的抗病品系在二片真叶展开2/3时,用高压喷雾接种(Xanthomonas campestris pv.glycines),接种后的第3、6、9天分别取样;样本分二种处理分别在扫描电镜、透射电镜和普通光学显微镜下观察病斑形成过程.接种后3天,肉眼可见褪绿斑时,病斑部分的表皮细胞和薄壁细胞组织已开始膨大,病斑部分稍有隆起.第6天,肉眼能见疱斑时,表皮细胞和薄壁细胞膨大明显,少数细胞开始破裂.第9天病斑开始变褐,电镜下可见疱斑完全破裂,表皮细胞和薄壁细胞膨大数倍于正常细胞,且细胞破裂,大量细菌溢出。同时可以看到大豆斑疹病细菌在发病初期是在细胞间隙中存活和繁殖的,在表皮细胞和薄壁细胞破裂后进入细胞内,最后完全破坏细胞.从电镜切片中也可以看到疱斑形成主要是由于细胞体积增大所致.抗病品种只有褪绿斑,渐变褐,肉眼看不见疱斑,但在电镜下可见得下表皮稍隆起,有少数细菌存在于细胞间隙,未见有细菌溢出现象。
Soybean spot disease caused by (Xanthomonas campestris pv.glycines) bulge bulge. Pot-soybean susceptible cultivars (Chip-pewa and Horosoy) and their resistant lines were seeded on top of two true leaves at 2/3 with high pressure spray (Xanthomonas campestris pv. Glycines) Day respectively.The sample was divided into two kinds of treatment to observe the formation of the lesion under the scanning electron microscope, the transmission electron microscope and the ordinary light microscope respectively.3 days after the inoculation, when the faded green spot was observed with the naked eye, the epidermic cells and the parenchyma cells On the sixth day, epidermal cells and parenchyma cells were swollen obviously, and a few cells began to rupture. On the 9th day, the lesion began to brown, and blister spots were observed under electron microscope Complete rupture, epidermal cells and parenchyma cells several times larger than normal cells, and the cells rupture, a large number of bacteria overflow. At the same time, it can be seen that during the early stages of the disease, the bacteria of soybean blight survive and multiply in the intercellular space, enter into the cells after the epidermal cells and the parenchyma cells rupture, and finally completely destroy the cells. Formation is mainly due to cell volume caused by the resistant varieties only chlorotic spot, brown gradient, the naked eye can not see blister spots, but the epidermis slightly elevated under electron microscopy, a small number of bacteria present in the intercellular space, there is no Bacteria overflow phenomenon.