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AIM:To explore the possible mechanism of intrauterineinfection of hepatitis B virus (HBV).METHODS:HBV DNA was detected in vaginal secretionand amniotic fluid from 59 HBsAg-positive mothers and invenous blood of their newborns by PCR.HBsAg and HBcAgin placenta were determined by ABC immunohistochemistry.RESULTS:The rate of HBV intrauterine infection was 40.1%(24/59).HBV DNA was detected in 47.5% of amniotic fluidsamples and 52.5% of vaginal secretion samples respectively.HBsAg and HBcAg were detected in placentas from HBsAg-positive mothers.The concentration of the two antigensdecreased from the mother’s side to the fetus’s side,in thefollowing order:maternal decidual cells > trophoblastic cells>villous mesenchymal cells > villous capillary endothelialcells.However,in 4 placentas the distribution was in thereverse order.HBsAg and HBcAg were detected in amnioticepithelial cells from 32 mothers.CONCLUSION:The main route of HBV transmission frommother to fetus is transplacental,from the mother side ofplacenta to the fetus side.However,HBV intrauterineinfection may take place through other routes.
AIM: To explore the possible mechanism of intrauterine infection of hepatitis B virus (HBV). METHODS: HBV DNA was detected in vaginal secretion and amniotic fluid from 59 HBsAg-positive mothers and invenous blood of their newborns by PCR. HBsAg and HBcAgin placenta were determined by ABC immunohistochemistry. RESULTS: The rate of HBV intrauterine infection was 40.1% (24/59). HBV DNA was detected in 47.5% of amniotic fluid samples and 52.5% of vaginal secretion samples respectively. HBsAg and HBcAg were detected in placentas from HBsAg-positive mothers. The concentration of the two antigens decreased from the mother’s side to the fetus’s side, in the following order: maternal decidual cells> trophoblastic cells> villous mesenchymal cells> villous capillary endothelial cells. Despite, in 4 placentas the distribution was in thereverse order. HBsAg and HBcAg were detected in amnioticepithelial cells from 32 mothers. CONCLUSION: The main route of HBV transmission from to fetus is transplacental, from the mo ther side of placenta to the fetus side. However, HBV intrauterine infection may take place through other routes.