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目的:研究硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞、SGC7901胃癌细胞及海拉细胞(Hela cells)内游离Ca2+的影响。方法:分离制备小鼠腹腔巨噬细胞,利用Ca2+荧光探针Fluo-3/AM及激光共聚显微镜测定加药后10 min细胞内Ca2+浓度的变化。结果:添加硒化多糖后,巨噬细胞内Ca2+浓度缓慢升高,而胃癌细胞与Hela细胞内Ca2+水平均有不同程度的下降。结论:通过对乳酸菌胞外多糖进行硒化修饰可增强其刺激巨噬细胞的能力,因而发挥其免疫功效。硒化多糖与肿瘤细胞表面受体结合后引起细胞内Ca2+内流的减少,提示硒化后的多糖可引起肿瘤细胞不同程度的细胞凋亡。
Objective: To study the effect of Lactobacillus selenarius exopolysaccharide on intracellular free Ca2 + in mouse peritoneal macrophages, SGC7901 gastric cancer cells and Hela cells. Methods: Mouse peritoneal macrophages were isolated and cultured. Fluorescence probe Fluo-3 / AM and laser scanning confocal microscope were used to detect the intracellular Ca2 + concentration at 10 min after drug addition. Results: After adding selenium polysaccharide, the Ca2 + concentration in macrophages increased slowly, while the intracellular Ca2 + levels in gastric cancer cells and Hela cells decreased to some extent. Conclusion: The lactic acid bacteria exopolysaccharide modified selenium can enhance its ability to stimulate macrophages, thus exerting its immune function. Selenium polysaccharide and tumor cell surface receptor binding caused by intracellular Ca2 influx decreased, suggesting that selenium polysaccharide can cause different degrees of tumor cell apoptosis.