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血清甲胎蛋白(AFP)的测定对于原发性肝癌的诊断有重大价值,但该试验有10～20%的假阴性,在少数肝炎等非肝癌患者时又可有假阳性。因此寻找新的生化试验以补充AFP测定的不足殊为必要。我们曾探讨过血清酶谱对肝病的诊断价值,发现γ-谷氨酰转移酶(GGT)和碱性磷酸酶(ALP)总活力的测定对原发性肝癌的诊断有一定帮助,但与其他肝病,尤其是伴有胆汁郁积的病例之间重叠性甚大,特异性不强。1967年Orlowski等应用淀粉胶电泳发现原发性肝癌病人血清自蛋白后面有一强染的GGT活性带,嗣后,一些学者相继应用聚丙烯酰胺凝胶电泳证实了所谓“肝癌特异性GGT”的存
The determination of serum alpha-fetoprotein (AFP) is of great value in the diagnosis of primary liver cancer, but the test has a false negative of 10-20%, and it may have false positives in a small number of non-hepatoma patients such as hepatitis. Therefore, it is necessary to look for new biochemical tests to supplement the deficiency of AFP measurement. We have explored the diagnostic value of serum zymography in liver disease and found that the determination of the total viability of gamma-glutamyltransferase (GGT) and alkaline phosphatase (ALP) may be helpful in the diagnosis of primary liver cancer, but with other Liver diseases, especially those associated with cholestasis, are highly overlapped and specificity is not strong. In 1967 Orlowski et al. applied starch gel electrophoresis to find that there was a strongly stained GGT active band behind the auto-protein in patients with primary liver cancer. Later, some scholars have applied polyacrylamide gel electrophoresis to confirm the so-called “hepatoma-specific GGT.” Save