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为研究饲料中添加β-葡聚糖对黄颡鱼幼鱼生长性能、血清生化指标及抗嗜水气单胞菌感染能力的影响,在基础饲料中分别添加200、600和1 000 mg/kgβ-葡聚糖配制3种试验饲料。选取初始体重为(1.53±0.04)g的健康黄颡鱼幼鱼720尾,随机分为7组,包括1个投喂基础饲料的对照组(G0组)、3个连续投喂添加β-葡聚糖饲料的连续投喂组(SG200、SG600、SG1000组)及3个间隔投喂添加β-葡聚糖饲料和基础饲料各7 d的间隔投喂组(IG200、IG600、IG1000组)。连续投喂组每组4个重复,对照组和间隔投喂组每组3个重复,每个重复均为30尾鱼。试验期为42 d。养殖试验结束后,注射嗜水气单胞菌进行感染试验,计算96 h内的累计死亡率。结果显示:1)7组黄颡鱼幼鱼的增重率、饲料系数、蛋白质效率和存活率均没有显著差异(P>0.05),但IG1000组的肝体比较对照组显著降低(P<0.05)。各β-葡聚糖添加组的增重率和蛋白质效率与对照组相比均有所升高,且连续投喂组的增重率高于对应的间隔投喂组,并在SG600组达到最高。2)各β-葡聚糖添加组全鱼粗蛋白质、粗脂肪和粗灰分含量均高于对照组,同时水分含量均低于对照组,但组间差异不显著(P>0.05)。3)饲料中添加β-葡聚糖对黄颡鱼幼鱼各血清生化指标均无显著影响(P>0.05);与对照组相比,SG1000和IG600组血清球蛋白和总蛋白含量有所升高,各β-葡聚糖添加组血清谷丙转氨酶和谷草转氨酶活性均有所降低。4)嗜水气单胞菌感染96 h后,各组的累计死亡率在35%~46%之间,组间差异不显著(P>0.05)。由此得出,除间隔投喂1 000 mg/kgβ-葡聚糖显著降低肝体比外,不论采取连续投喂还是间隔投喂方式,饲料中添加200、600和1 000 mg/kgβ-葡聚糖对黄颡鱼幼鱼的生长性能、体成分、血清生化指标及抗嗜水气单胞菌感染能力均没有显著影响。
In order to study the effects of β-glucan on the growth performance, serum biochemical indexes and the ability of anti-Aeromonas hydrophila infection of juvenile yellow catfish, 200,600 and 1,000 mg / kgβ - Dextran formulated three kinds of test feed. A total of 720 juvenile yellow catfish with initial body weight of (1.53 ± 0.04) g were randomly divided into seven groups, including one control group (group G0) fed basal diet and three (SG200, SG600, SG1000 group) and three fed-fed groups (IG200, IG600, IG1000) fed with β-glucan and basal diet for 7 days respectively. The rats in the continuous feeding group had 4 replicates in each group, 3 replicates in the control group and the intermittent feeding group with 30 fish in each replicate. The test period is 42 days. After the end of the aquaculture test, A. hydrophila was injected to test the cumulative mortality within 96 h. The results showed that: 1) There was no significant difference in weight gain rate, feed efficiency, protein efficiency and survival rate between the 7 groups of juvenile yellow catfish (P> 0.05), but the IG1000 group was significantly lower than the control group (P <0.05 ). The weight gain rate and protein efficiency of each β-glucan addition group increased compared with the control group, and the weight gain rate of the continuous feeding group was higher than that of the corresponding interval fed group and reached the highest in the SG600 group . 2) The content of crude protein, crude fat and crude ash of the whole fish in the β-glucan addition group were higher than those in the control group, while the moisture content was lower than the control group, but the difference was not significant (P> 0.05). Serum globulin and total protein in SG1000 and IG600 groups increased compared with the control group (P> 0.05) .3) The addition of β-glucan to feed did not significantly affect the serum biochemical indexes of juvenile yellow catfish (P> 0.05) High, the β-glucan added serum alanine aminotransferase and aspartate aminotransferase activity were reduced. 4) Aeromonas hydrophila infection 96 h, the cumulative mortality in each group was between 35% to 46%, the difference between the groups was not significant (P> 0.05). It was concluded that in addition to intermittent feeding of 1 000 mg / kg β-glucan significantly reduced liver body ratio, regardless of continuous feeding or interval feeding mode, the feed added 200,600 and 1 000 mg / kg β-glucose Glycan had no significant effect on the growth performance, body composition, serum biochemical index and anti-Aeromonas hydrophila infection of juvenile yellow catfish.