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目的:建立白花丹中β-谷甾醇含量的HPLC-ELSD快速测定方法,比较野生与家种药材中β-谷甾醇的含量。方法:采用HPLC-ELSD法测定野生与家种白花丹药材根、茎、叶中β-谷甾醇含量。色谱条件为:色谱柱为迪马C_(18)柱(250mm×4.6mm,5μm);流动相为纯甲醇;流速为1.0mL/min;柱温:25℃;以蒸发光散射检测器进行检测,检测参数为漂移管温度65℃,载气(N2)流速为2.5L/min。结果:β-谷甾醇在0.2~3.5μg/mL范围内与峰面积值呈良好线性关系,回归方程为:Y=1.500 3X+5.582 6;β-谷甾醇在叶中含量最高。结论:以β-谷甾醇为主要活性成分时最佳入药部位为叶,白花丹叶为可再生资源,可作为β-谷甾醇的新药用资源植物。
OBJECTIVE: To establish a HPLC-ELSD rapid determination method for the content of β-sitosterol in Chinese white Huadan, and to compare the content of β-sitosterol in wild and domestic medicinal materials. Methods: The contents of β-sitosterol in roots, stems and leaves of wild and home-grown Chinese white Huadan herbs were determined by HPLC-ELSD. The chromatographic conditions were as follows: the column was Dima C 18 column (250mm × 4.6mm, 5μm); the mobile phase was pure methanol; the flow rate was 1.0mL / min; the column temperature was 25 ℃; the detection was performed by evaporative light scattering detector , The detection parameters for the drift tube temperature 65 ℃, carrier gas (N2) flow rate of 2.5L / min. Results: There was a good linear relationship between β-sitosterol and peak area in the range of 0.2-3.5 μg / mL. The regression equation was: Y = 1.500 3X + 5.582 6; β-sitosterol was the highest in leaf. CONCLUSION: The best medicine site is β-sitosterol as the main active ingredient, while the white-leaf dandelion is a renewable resource, which can be used as a new medicinal resource of β-sitosterol.