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对在黑龙江哈尔滨闫家岗农场采集的绥农14大豆细菌性斑点病叶片进行病原菌分离,将分离得到的大豆细菌性斑点病病原菌进行抗生素耐性鉴定、分子鉴定并对大豆品种回接试验,分离得到1个细菌菌株。经过革兰氏染色、病原菌回接试验、16S r DNA分子鉴定方法确定分离的菌株为丁香假单胞杆菌属,命名为Psgneau 2。分离的菌株对终浓度为25,50,100μg·m L~(-1)的氨苄青霉素和羧苄青霉素都具有耐性,对壮观青霉素、利福平、卡那霉素、庆大霉素、四环素和氯霉素不具有抗性。采用高压喷雾和菌落滴度的方法将分离的Psgneau 2菌株接种10个大豆品种,进行致病性鉴定,结果表明绥农14极易感病,合丰35抗病性高于其它品种。
The pathogen was isolated from the bacterial leaf spot of Suinong 14 soybeans collected in Yanjigang Farm, Harbin, Heilongjiang Province. The pathogen was isolated from soybean bacterial speck pathogen for identification of antibiotic resistance, molecular identification and backtesting of soybean varieties. One Bacterial strains. After Gram stain, pathogen back connection test, 16S r DNA molecular identification method to determine the isolated strain of Pseudomonas syringae, named Psgneau 2. The isolated strains were resistant to ampicillin and carbenicillin at the final concentration of 25, 50, 100μg · m L -1. The isolates were resistant to spectinomycin, rifampicin, kanamycin, gentamicin, tetracycline and Chloramphenicol is not resistant. The isolated Psgneau 2 strain was inoculated with 10 soybean varieties by high-pressure spray and colony titer, and pathogenicity identification was carried out. The results showed that Suinong 14 was extremely susceptible to disease and Hefeng 35 had higher disease resistance than other varieties.